目的 研究醋酸齊考諾肽重復(fù)鞘內(nèi)注射給藥的局部刺激性，為鞘內(nèi)注射制劑的局部刺激性評(píng)價(jià)提供參考。方法 16只新西蘭兔，隨機(jī)分為對(duì)照組（生理鹽水）和醋酸齊考諾肽組，每組8只，雌雄各半，腰椎穿刺鞘內(nèi)注射給藥，給藥濃度為100 μg/mL，體積為50 μL/只，每天1次，連續(xù)給藥7 d，恢復(fù)14 d。每天觀察動(dòng)物一般癥狀，并于末次給藥后2 d，每組處死4只動(dòng)物進(jìn)行剖檢，取注射部位及前段和后段處腰椎和脊髓進(jìn)行組織病理學(xué)檢查及刺激性評(píng)價(jià)，剩余動(dòng)物于末次給藥后14 d剖檢并進(jìn)行刺激性評(píng)價(jià)。結(jié)果 對(duì)照組1只動(dòng)物于給藥第3天麻醉后死亡，其余動(dòng)物在給藥及恢復(fù)期間未見(jiàn)明顯異常癥狀；末次給藥后2、14 d，各組動(dòng)物組織病理學(xué)檢查均未見(jiàn)明顯的鞘內(nèi)刺激性反應(yīng)；末次給藥后2 d，注射點(diǎn)見(jiàn)可逆的輕微機(jī)械性損傷。結(jié)論 本試驗(yàn)條件下，與對(duì)照組比較，未見(jiàn)醋酸齊考諾肽對(duì)腰椎、硬脊膜和脊髓的局部刺激性作用，新西蘭兔可作為鞘內(nèi)注射制劑的局部刺激性評(píng)價(jià)模型。

Objective To study the local irritation of repeated intrathecal injection of Ziconotide Acetate, and to provide reference for irritancy evaluation of intrathecal injection. Methods Sixteen New Zealand rabbits were assigned into two groupsat random:Control group and Ziconotide Acetate group, eight animals each group. Totally 50 μL saline or Ziconotide Acetate (100 μg/mL) were administrated by repeated lumbar intrathecal injection once daily for 7 d. Animal behavior was observed every day, and four animals in each group were sacrificed 2 d later after the last injection, the lumbar spinal cord was removed for histopathological examination and irritancy evaluation. The remaining animals were sacrificed for irritancy evaluation 14 d later after the last injection. Results Only one animal died after anesthesia on day three in saline group, while no obvious adverse reactions were observed in other rabbits during the entire study, and no intrathecal irritant reactions of histopathological examination were found in both groups. The reversible minor mechanical damage was observed at the injection point, 2 d after the last administration. Conclusion For 7 d repeated lumbar intrathecal injection in rabbits, no intrathecal irritant reactions observed in Ziconotide group, and the New Zealand rabbit could be used as a local irritation evaluation model.