目的 建立反相高效液相色譜測(cè)定強(qiáng)力枇杷露中磷酸可待因的方法。方法 采用Eclipse XDB-C₁₈柱（250 mm×4.6mm，5 μm），乙腈-0.035 mol/L醋酸鈉溶液（冰醋酸調(diào)pH＝3.5）（25∶75）為流動(dòng)相，體積流量為1.0 mL/min，檢測(cè)波長(zhǎng)為240 nm，柱溫為40℃。結(jié)果 磷酸可待因在2.024~40.24 μg/mL線性良好，回歸方程為y＝8.18×105x＋12.2（r＝0.999 2）；平均加樣回收率為98.2%，RSD為1.0%（n＝6）。結(jié)論 該方法快速準(zhǔn)確，靈敏度高，用于強(qiáng)力枇杷露中鹽酸可待因含量測(cè)定，控制藥品質(zhì)量。

Objective To establish the determination method of Codeine phosphate in Qiangli Pipa Syrup by RP-HPLC. Methods Using XDB-C₁₈ Eclipse column (250 mm × 4.6 mm, 5 ìm), acetonitrile: 0.035 mol/L sodium acetate solution (glacial acetic acid to adjust pH = 3.5) (25: 75) as mobile phase, the flow rate was 1.0 mL/min, the detection wavelength is 240 nm, the column temperature is 40. Results the linear range of codeine phosphate in 2.024—40.24 ìg/mL was good y = 8.18 × 10⁵ x + 12.2 (r = 0.999 2); the average recovery rate was 98.2% and RSD was 1.0% (n = 6). Conclusion This method is simple, rapid, accurate, and reliable, with high sensitivity, and could be applied to determination of Qiangli Pipa Syrup codeine hydrochloride content and controlment of the drug quality.