目的 研究不同產(chǎn)地枇杷葉醇提物對(duì)抗炎靶標(biāo)磷酸二酯酶4（PDE4）的抑制作用。方法 采用體積分?jǐn)?shù)95%乙醇回流提取法提取藥材，以標(biāo)記液體閃爍計(jì)數(shù)法檢測(cè)提取物對(duì)PDE4的D2亞型靶標(biāo)（PDE4D2）的抑制活性。結(jié)果 測(cè)試體系的藥物終質(zhì)量濃度為5 mg/L時(shí)，20個(gè)樣品中9個(gè)測(cè)試樣品的抑制率在80%以下；11個(gè)樣品的抑制率大于80%，其中8個(gè)樣品大于90%。結(jié)論 不同地枇杷葉醇提物抑制PDE4靶標(biāo)的活性存在明顯差異。

Objective To investigate the inhibition of Eriobotryae Folium from twenty different districts towards phosphodiesterase 4(PDE4) in vitro. Methods The Eriobotryae Folium were extracted with 95% ethanol reflux and the inhibition rates against PDE4D2 were carried out by liquid scintillation counting method. Results All the samples exhibited inhibitory activities towards PDE4 at 5 mg/L. Among them, nine samples were of the inhibition rate less than 80%, eleven samples were of more than 80% inhibition and eight samples were of more than 90% inhibition. Conclusion The Eriobotryae Folium shows significantly different inhibitory activities towards PED4.

國(guó)家自然科學(xué)基金項(xiàng)目（21103234，81373258）；廣東自然科學(xué)基金團(tuán)隊(duì)項(xiàng)目（S2011030003190）；廣州市科技計(jì)劃項(xiàng)目（201607010293，2014J4100165）；國(guó)家公益性行業(yè)專(zhuān)項(xiàng)（201407002）