目的 探討莪術(shù)油對(duì)直腸癌SW1463細(xì)胞株增殖、凋亡及相關(guān)蛋白Caspase-3、Bax、Bcl-2表達(dá)的影響。方法 水蒸氣蒸餾法提取黔產(chǎn)莪術(shù)揮發(fā)油，配制成40、80、120、160、200、240、280 mg/L濃度梯度，干預(yù)SW1463細(xì)胞24、48、72 h，MTT法檢測(cè)莪術(shù)油對(duì)SW1463細(xì)胞的增殖抑制率；Giemsa染色法觀察莪術(shù)油對(duì)SW1463細(xì)胞凋亡形態(tài)的影響；蛋白免疫印跡法（Western blotting）檢測(cè)Capase-3、Bax與Bcl-2蛋白表達(dá)。結(jié)果 莪術(shù)油對(duì)SW1463細(xì)胞的增殖有明顯抑制作用，并呈現(xiàn)時(shí)間-劑量相關(guān)性，24、48、72 h的半數(shù)抑制濃度（IC₅₀）分別為144.33、134.11、120.04 mg/L；Giemsa染色可見(jiàn)細(xì)胞明顯的凋亡形態(tài)學(xué)特征；莪術(shù)油干預(yù)SW1463細(xì)胞24 h后，與對(duì)照組比較，Caspase-3、Bax蛋白表達(dá)顯著上調(diào)、Bcl-2蛋白表達(dá)顯著下調(diào)（P<0.05）。結(jié)論 莪術(shù)油能明顯抑制SW1463細(xì)胞增殖，誘導(dǎo)細(xì)胞凋亡，其機(jī)制可能與上調(diào)Caspase-3和Bax蛋白表達(dá)、下調(diào)Bcl-2蛋白表達(dá)相關(guān)。

Objective To explore the effects of zedoary turmeric oil on proliferation and apoptosis of SW1463 cell line and the expression of Caspase-3, Bax and Bcl-2.Methods Volatile oil from Curcumae Rhizoma in Guizhou was extract by steam distillation, which was used to intervene SW1463 cells for 24, 48 and 72 h at concentration of 40, 80, 120, 160, 200, 240 and 280 mg/mL. MTT method was used to detect the inhibitory rate of zedoary turmeric oil on SW1463 cell proliferation. Effects of different concentrations of zedoary oil on apoptosis of SW1463 cells were observed by Giemsa staining. Western blotting was used to detect Capase-3, Bax and Bcl-2 protein expression.Results Zedoary turmeric oil inhibited the proliferation of SW1463 cells and showed a time dose correlation, and half maximal inhibitory concentration (IC₅₀) of 24, 48 and 72 h was 144.33, 134.11 and 120.04 mg/L, respectively. Giemsa staining showed obvious morphological characteristics of apoptotic cells. Western blotting results showed that compared with control group, the expression of Caspase-3 and Bax in cells treated with zedoary turmeric oil for 24 h were significantly up-regulated (P<0.05), and the expression of Bcl-2 protein was significantly down-regulated (P<0.05).Conclusion Zedoary turmeric oil can obviously inhibit the proliferation of SW1463 cells and induce apoptosis, which may be related to the up-regulation of Caspase-3 and Bax protein expression and down-regulation of Bcl-2 protein expression.

貴州省廳校聯(lián)合項(xiàng)目[黔科合LG字（2011）006號(hào)]；貴州省社會(huì)發(fā)展項(xiàng)目[黔科合SY（2008）3027號(hào)]