目的 研究巴曲酶注射液對(duì)大鼠血栓栓塞性腦卒中急性超早期的保護(hù)作用。方法 自體血凝塊閉塞左側(cè)大腦中動(dòng)脈法制備大鼠血栓栓塞性腦卒中模型，32只造模成功大鼠按神經(jīng)缺陷程度隨機(jī)分為4組：模型組及巴曲酶注射液低、高劑量（0.3、1.0 BU/kg）組和阿替普酶（rt-PA，9 mg/kg）組，每組8只，另設(shè)假手術(shù)組8只。造模1 h后尾iv給藥，給藥后6 h行神經(jīng)功能評(píng)分，采用核磁共振（MIR）技術(shù)進(jìn)行大鼠腦SE-T2WI序列掃描，測(cè)量腦病變范圍；給藥后24 h評(píng)分后取腦進(jìn)行TTC染色，測(cè)量腦梗死范圍；給藥后6、24 h取血漿，測(cè)纖維蛋白原（FIB）濃度。結(jié)果 與模型組比較，巴曲酶注射液0.3 BU/kg治療24 h（P<0.05）、1 BU/kg治療6、24 h（P<0.05、0.01）顯著改善大鼠神經(jīng)功能評(píng)分；給藥后6 h MRI結(jié)果顯示，巴曲酶注射液0.3、1.0 BU/kg顯著縮小病變范圍（P<0.05、0.01）；給藥后24 h TTC結(jié)果顯示，巴曲酶注射液0.3、1.0 BU/kg顯著縮小梗死范圍（P<0.05）；巴曲酶注射液0.3、1.0 BU/kg于藥后6、24 h均可顯著降低血漿FIB濃度（P<0.05、0.01、0.001）。結(jié)論 巴曲酶注射液能改善大鼠腦缺血急性期受損神經(jīng)功能、縮小腦病變范圍、降低血漿FIB濃度，具腦保護(hù)作用。

Objective To observe the effects of Batroxobin Injection on thromboembolic cerebral stroke by magnetic resonance imaging (MRI) and TTC staining.Methods Rat model of thromboembolic stroke was prepared after the left middle cerebral artery was occluded by autologous blood clots, and 32 rats with successful operation were divided into four groups according to the degree of neurological deficit: model group, Batroxobin Injection low and high dose (0.3, 1.0 BU/kg) group, and rt-PA (9 mg/kg) group, with eight rats in each group, and other eight rats in Sham group. Rats were administered 1 h after modeling by tail iv method. At 6 h after administration, neurological deficit score and MRI including SE-T2WI and DWI sequence scanning were measured. At 24 h after administration, the brain was cut for TTC staining to measure the infarct area, and blood FIB was measured.Results Compared with model group, Batroxobin Injection 0.3 BU/kg treatment for 24 h (P<0.05), 1 BU/kg treatment for 6 and 24 h (P<0.05, 0.01) could significntly improve the neurological function scores of rats. MRI results showed that Batroxobin Injection at dose of 0.3 and 1 BU/kg significantly reduced the lesion range (P<0.05 and 0.01). Results of TTC stain showed that Batroxobin Injection at dose of 0.3 and 1 BU/kg significantly reduced the infarct size (P<0.05). Batroxobin Injection at doses of 0.3 and 1 BU/kg can significantly lower plasma FIB concentration (P<0.05, 0.01, 0.001) 6 and 24 h after administration.Conclusion Batroxobin Injection can improve the damaged neural function, reduce scope of lesions, decrease plasma fibrinogen, with protective effects for cerebral ischemia in rats.