目的 通過觀察元胡止痛滴丸及其組分對縮宮素痛經(jīng)模型的影響，并與臨床上同類藥月月舒對比，探討其配伍的合理性及比較優(yōu)勢。方法 通過ip縮宮素誘發(fā)大鼠痛經(jīng)模型，觀察元胡止痛滴丸及其組成成分延胡索浸膏、白芷浸膏對扭體反應(yīng)潛伏期及次數(shù)的影響；ELISA法檢測與疼痛相關(guān)血漿中5-羥色胺（5-HT）、去甲腎上腺素（NA）、β-內(nèi)啡肽（β-EP）、前列腺素F_2α（PGF_2α）、前列腺素E₂（PGE₂）水平，腦組織中多巴胺（DA）水平；金氏概率相加法計(jì)算Q值，根據(jù)Q值是否大于1判斷元胡止痛滴丸的效果是否優(yōu)于組分單用。結(jié)果 與模型組比較，元胡止痛滴丸、延胡索浸膏和白芷浸膏能夠顯著減少扭體次數(shù)、延長扭體反應(yīng)的潛伏期（P<0.05、0.01、0.001），Q值均大于1；與月月舒比較，元胡止痛滴丸作用顯著增強(qiáng)（P<0.05、0.01）。與模型組比較，元胡止痛滴丸和延胡索浸膏可顯著升高β-EP、PGE₂水平（P<0.05、0.01），顯著降低5-HT、NA、DA、PGF_2α水平及PGF_2α/PGE₂比值（P<0.05、0.01、0.001）；白芷浸膏可顯著降低5-HT、DA水平（P<0.05、0.01）；β-EP、NA、DA和PGE2的Q值均大于1；與月月舒比較，元胡止痛滴丸可顯著升高β-EP、PGE₂水平，顯著降低5-HT、PGF_2α水平及PGF_2α/PGE₂比值（P<0.05、0.01）。結(jié)論 元胡止痛滴丸對痛經(jīng)模型具有顯著的鎮(zhèn)痛作用，作用強(qiáng)度優(yōu)于組分單用，配伍具有合理性，與月月舒比較具有起效早、作用強(qiáng)的特點(diǎn)。

Objective To observe compatibility rationality and comparative advantage of analgesic effect of Yuanhu Zhitong Dropping Pills (YZDP) compared with its ingredient and Yueyueshu through dysmenorrhea model induced by oxytocin.Methods Using dysmenorrhea model induced by ip injection of oxytocin to observe the effect of YZDP, Corydalis extract and Angelica dahurica extract on the latency and times of writhing response, and ELISA method was used to test the level of factors associated with pain in plasma, including 5-serotonin (5-HT), norepinephrine (NA), β-endorphin (β-EP), prostaglandin F_2α (PGF_2α) and prostaglandin E₂ (PGE₂), and level of dopamine (DA) in brain tissue. Q value was calculated by prime probability addition method, and according to whether the Q value was greater than one to determine whether the effects of YZDP was better than that of single component.Results Compared with model group, YZDP, Corydalis extract and Angelica dahurica extract could significantly prolong the latency of writhing response, decrease writhing times (P<0.05, 0.01 and 0.001), and the Q values were greater than one. Compared with Yueyueshu, effect of Yuanhuzhitong dropping pills was significantly increased (P<0.05 and 0.01). YZDP and Corydalis extract could increase the level of β-EP as well as PGE₂, and decrease the level of 5-HT, NA, DA, PGF_2α and the ratio of PGF_2α to PGE₂ obviously in dysmenorrhea model rats (P<0.05, 0.01 and 0.001). A. dahurica extract can significantly reduce 5-HT and DA level (P<0.05, 0.01). Q values of β-EP, NA, DA and PGE₂ were greater than one. Compared with Yueyueshu, YZDP increased the levels of β-EP and PGE₂, and decreased the levels of 5-HT, PGF_2α and PGF_2α/PGE₂ significantly (P<0.05 and 0.01).Conclusion YZDP has significant analgesic effect on dysmenorrhea model and has compatibility rationality because of better analgesic effect than ingredient use only. Compared with Yueyueshu, YZDP has the characteristic of working early and better analgesic effect.

國家科技部“重大新藥創(chuàng)制”科技重大專項(xiàng)（No2015ZX09501004-002-003）