目的 考察大劑量ig輔酶Q10對小鼠中樞神經(jīng)系統(tǒng)的影響，為臨床安全性評價提供實驗依據(jù)。方法 將小鼠隨機分為溶媒對照組、紫蘇油對照組、陽性對照組（氯丙嗪或地西泮）和輔酶Q10低、中、高劑量組（1.5、3.0和6.0 g/kg，相當于臨床等效劑量的75、150和300倍），每組12只，雌雄各半，40 mL/kg單次ig給藥。直接觀察小鼠一般行為活動；轉(zhuǎn)棒法觀察小鼠運動協(xié)調(diào)能力；Anymaze動物行為學視頻分析系統(tǒng)觀察小鼠自發(fā)活動和與閾下劑量戊巴比妥鈉的協(xié)同作用。結(jié)果 與溶媒對照組、紫蘇油對照組比較，輔酶Q10 3個劑量組對小鼠一般行為活動、自發(fā)活動、運動協(xié)調(diào)能力和與閾下劑量戊巴比妥鈉的協(xié)同作用均無顯著差異。結(jié)論 大劑量ig輔酶Q10對小鼠中樞神經(jīng)系統(tǒng)未見明顯毒性作用。

Objective To investigate the effect of high-dose coenzyme Q10 on the central nervous system in mice, and to provide experimental basis for clinical safety evaluation.Methods Mice were randomly divided into vehicle control group, perillartine control group, positive control group (chlorpromazine or diazepam) and coenzyme Q10 low, medium and high dose groups (1.5, 3.0 and 6.0 g/kg, equivalent to 75, 150, and 300 times of clinical dosage, respectively). The corresponding drugs were ig given to mice with the volume of 40 mL/kg. The general behavior of mice was observed directly, the motor coordination ability was observed by rotating stick method, and Anymaze animal behavior video analysis system was used to observe the spontaneous activity of mice and synergistic reaction with sub-threshold dose of pentobarbital sodium.Results There were no significant differences in the general behavioral activity, and the number of spontaneous activity times, mean resident time, and ratio of sleeping were found in all coenzyme Q10 groups, compared with the vehicle and perillartine control groups.Conclusion High dose of coenzyme Q10 has no significantly toxic effect on the central nervous system in mice, which could provide a reliable experimental basis for further medication study and clinical application of high-dose coenzyme Q10.