50,受精后3 d)約373 μg/mL,半數(shù)致死濃度(LD50)約為485 μg/mL。濃度為1 200 μg/mL時,胚胎在受精后1 d之內(nèi)全部死亡。結(jié)論 牛黃鎮(zhèn)驚丸給藥濃度大于200 μg/mL時影響斑馬魚胚胎發(fā)育。其毒性作用發(fā)生在胚胎器官形成期,主要對胚胎的神經(jīng)系統(tǒng)、心血管系統(tǒng)和骨骼等發(fā)育有影響。;Objective To study the toxic effect of Niuhuang Zhenjing Pill on zebrafish embryo development.Methods Zebrafish embryo was used to investigate the development situation under stereo microscope at different drug concentration (40, 200, 400, 800, 1 200 μg/mL) including embryo teratogenic and lethal tests.Results No abnormal embryo development was found when the concentration was not greater than 200 μg/mL (Approximately equal to 30 times the normal adult dosage, exceeding the normal effective dose). While, there was toxicity to embryo development at the concentration greater than 200 μg/mL. The head and eyes of the embryo were smaller, and the pupils were colorless. Zebrafish embryonic heart malformation was observed, heart rate was weak, blood pool congestion happened and back blood flow was not smooth. The abdominal surface of the embryo was poor in transparency, the tail was twisted, the yolk sac elongation structure became thicker, the notochord became thin, there was no normal embryo movement, and the body length became shorter. After the withdrawal, the abnormal phenotype was not recovered, and severity of the abnormal phenotype was concentration-dependent. It was also observed that all embryos died within 1 d after fertilization at the concentration of 1 200 μg/mL.Conclusion The drug could influence the development of zebrafish embryos when the concentration was higher than 200 μg/mL. Its toxic effect occurs during embryonic organogenesis. And it mainly affects the development of embryonic nervous system, cardiovascular system and bone."/>

醉酒后少妇被疯狂内射视频,一本色道久久综合一,在线天堂新版资源www在线下载,中文字幕乱人伦高清视频,中字幕视频在线永久在线观看免费

首頁 > 過刊瀏覽>2017年第40卷第7期 >2017,40(7):931-934. DOI:10.7501/j.issn.1674-6376.2017.07.009
上一篇 | 下一篇

牛黃鎮(zhèn)驚丸對斑馬魚胚胎發(fā)育的影響

Toxic effect of Niuhuang Zhenjing Pill on zebrafish embryo development

發(fā)布日期:2017-08-09
您是第位訪問者
藥物評價研究 ® 2025 版權(quán)所有
技術(shù)支持:北京勤云科技發(fā)展有限公司
津備案:津ICP備13000267號 互聯(lián)網(wǎng)藥品信息服務(wù)資格證書編號:(津)-非經(jīng)營性-2015-0031