目的 建立一測多評法檢測華佗豆藥材中4種生物堿的量。方法 以華佗豆堿乙為內(nèi)標(biāo)，建立華佗豆堿甲、堿丙、堿丁的相對校正因子，在考察相對校正因子的重現(xiàn)性后，進(jìn)一步采用外標(biāo)法與一測多評法所計(jì)算出的成分含量進(jìn)行比較。結(jié)果 6批華佗豆藥材中，各成分采用相對因子計(jì)算的量與采用外標(biāo)法計(jì)算的結(jié)果無顯著差異。結(jié)論 一測多評法操作簡單、結(jié)果準(zhǔn)確、可靠，可用于華佗豆4種生物堿的測定。

Objective To develop a method of quantitative analysis of multi-components by single marker (QAMS) for simultaneously determining four compounds in Calonyction muricatum.Method An HPLC method was developed as QAMS to determine ipalbidine, ipalbidinium, and ipalbinium in C. muricatum, using ipalbine as the intermal reference substance, the relative correction factor (RCF) of the three components was determined by HPLC with good reproducibility. Their contents in six batches of samples were determined by both external standard method and QAMS.Result No significant differences were found in the quantitative results of three alkaloid compounds in six batches of C. muricatum determined by external standard method and QAMS.Conclusion It is feasible and suitable to evaluate the quality of curcuma aromatic by QAMS.