2 column (250 mm×4.6 mm, 5 μm) was used. The mobile phase was acetonitrile-water (70:30). The flow rate was 1.0 mL/min. The column temperature was 45 ℃, the detector temperature was 40 ℃ and the injection volume was 10 μL.Results Lactose showed good linearity in the range of 0.2—5.0 mg/mL (r≥0.999 8). The detection limit was 0.06 mg/mL. The average recovery was 99.6% and the relative standard deviation was 0.7%.Conlusion The method is specific, convenient, fast and accurate, which can be used for determination of accessories lactose."/>

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首頁(yè) > 過(guò)刊瀏覽>2017年第40卷第7期 >2017,40(7):942-945. DOI:10.7501/j.issn.1674-6376.2017.07.012
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HPLC-RID法測(cè)定輔料乳糖

Determination of accessories lactose by HPLC-RID

發(fā)布日期:2017-08-09
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