2 column (250 mm×4.6 mm, 5 μm) was used. The mobile phase was acetonitrile-water (70:30). The flow rate was 1.0 mL/min. The column temperature was 45 ℃, the detector temperature was 40 ℃ and the injection volume was 10 μL.Results Lactose showed good linearity in the range of 0.2—5.0 mg/mL (r≥0.999 8). The detection limit was 0.06 mg/mL. The average recovery was 99.6% and the relative standard deviation was 0.7%.Conlusion The method is specific, convenient, fast and accurate, which can be used for determination of accessories lactose."/>