目的 建立同時(shí)測(cè)定芍藥苷、阿魏酸、蕓香柚皮苷、柚皮苷、新橙皮苷、甘草酸含量的超高效液相色譜（UPLC）法，研究柴胡疏肝散水提物的腸吸收特性。方法 采用大鼠在體單向腸灌流和外翻腸囊實(shí)驗(yàn)?zāi)Ｐ?，?yīng)用UPLC法測(cè)定柴胡疏肝散水提物中指標(biāo)性成分（芍藥苷、阿魏酸、蕓香柚皮苷、柚皮苷、新橙皮苷和甘草酸）在不同時(shí)間點(diǎn)、不同部位腸道吸收量，計(jì)算吸收動(dòng)力學(xué)參數(shù)，考察其腸吸收特征。結(jié)果 在體單向腸灌流模型結(jié)果表明，芍藥苷、蕓香柚皮苷、新橙皮苷、柚皮苷和甘草酸均為中等程度吸收的化學(xué)成分，而阿魏酸為完全吸收；外翻腸囊模型結(jié)果表明，芍藥苷在空腸部位，阿魏酸、柚皮苷和新橙皮苷在十二指腸部位，蕓香柚皮苷在十二指腸和空腸部位的吸收最佳，而甘草酸在各腸段的吸收無顯著性差異。結(jié)論 腸道對(duì)柴胡疏肝散中6種指標(biāo)性成分均有吸收，阿魏酸較其他5種指標(biāo)性成分更易透過腸壁進(jìn)入血液循環(huán)；不同腸段對(duì)6種指標(biāo)性成分的吸收具有選擇性。

Objective To evaluate the intestinal absorption of main components (paeoniflorin, ferulic acid, narirutin, naringin, neohesperidin, and glycyrrhizic acid) in aqueous extract of Chaihu Shugan San (CSS). Methods UPLC method was established to simultaneously determine the concentrations of the six components in the test samples of everted gut sacs and in situ single pass intestinal perfusion (SPIP) models. Absorption kinetics parameters were calculated for describing their absorption characteristics. Results In SPIP model, the results indicated that ferulic acid was the well-absorbed ingredient in whole small intestine, while other ingredients presented moderate or poor absorption. In everted gut sacs model, paeoniflorin in jejunum, ferulic acid, naringin, and neohesperidin in duodenum, narirutin in duodenum and jejunum, had the best absorption, while there was no significant difference in absorption of glycyrrhizic acid in the intestine. Conclusion In vivo model indicated that the main constituents in CSS could be absorbed in intestinal wall of rat, ferulic acid could be much more easily penetrated intestinal wall into the blood circulation than the other five components. Ex vivo model could further articulate that six index components could be absorbed selectively in different intestinal segments.

國(guó)家自然科學(xué)基金項(xiàng)目（NO.81673584）