目的 探討柴枳夏及湯對實(shí)驗(yàn)性膽汁反流性胃炎（BRG）大鼠胃黏膜的保護(hù)作用及機(jī)制。方法 將Wistar大鼠按照體質(zhì)量及性別以隨機(jī)數(shù)字表法分為5組：對照組、模型組、鋁碳酸鎂片（陽性藥）組和柴枳夏及湯低、高劑量（生藥量12.77、25.54 g/kg）組，除對照組外，ig自制反流液制備大鼠BRG模型。觀察對照組及模型組大鼠進(jìn)食、大小便、毛色、體質(zhì)量等；肉眼及HE染色后于光鏡下觀察胃竇黏膜病理組織學(xué)變化；ELISA法檢測血清GAS及胃竇黏膜PGE2水平。結(jié)果 與對照組比較，模型組大鼠出現(xiàn)排稀糊便、便中含紅黃色黏液、反應(yīng)遲鈍、體質(zhì)量顯著下降（P<0.05）等特點(diǎn)；肉眼可見胃竇部點(diǎn)片狀糜爛，有黃綠色膽汁及較多黃色黏液；胃竇黏膜出現(xiàn)大量炎性細(xì)胞浸潤和腸上皮化生，炎癥及腸上皮化生積分顯著提高（P<0.01）；GAS及PGE2水平明顯下降（P<0.01）；與模型組比較，柴枳夏及湯高劑量明顯改善胃黏膜病理組織損傷形態(tài)；減輕炎性細(xì)胞浸潤和腸上皮化生，顯著降低積分（P<0.05、0.01）；顯著提高GAS及PGE2水平（P<0.01）。結(jié)論 柴枳夏及湯具有明顯的保護(hù)胃黏膜的作用，其機(jī)制可能與調(diào)節(jié)血清GAS、胃竇黏膜PGE2水平有關(guān)。

Objective To investigate the therapeutic effect and mechanism of Chaizhixiaji Decoction on experimental bile reflux gastritis (BRG) rats. Methods Wistar rats were randomly divided into five groups by random according to weight and sex:control group, model group, Chaizhixiaji Decoction low and high dose groups, and Hydrotalcite Tablets group. Rats were given self-made reflux liquid orally to induce experimental bile reflux gastritis. The diet, stool and urine, hair color and body weight of rats in control group and model group were observed. The histopathological changes of gastric antrum mucosa were observed by naked eyeand light microscope after HE staining. ELISA method was used for detection of serum GAS, PGE2 content in gastric antrum mucosa. Result Compared with control group, rats in model group show thin boicing stool containing red yellow mucus, slow reaction, and body weight decreased significantly (P<0.05). There were patchy erosions of the gastric antrum with yellow green bile and more yellow mucus of model group in the naked eye. Model control group had a significant rise of inflammatory cells infiltration and intestinal metaplasia, and scores of inflammation and intestinal metaplasia increased significantly (P<0.01). GAS and PGE2 contents obviously decreased compared with that in control group (P<0.01). Compare with model group, Chaizhixiaji Decoction of high dosesignificantly improved gastric mucosal tissue damage morphology; reduced the infiltration of inflammatory cells and intestinal metaplasia, which score decreased significantly (P<0.05, 0.01); and increasedlevels of GAS and PGE2 significantly (P<0.01). Conclusion Chaizhixiaji Decoction has obvious protective effect on gastric mucosa, and its mechanism may be related with the regulation of GAS, PGE2 contents.