目的 探討姜黃素對小鼠皮膚移植排斥反應的免疫抑制作用。方法 BALB/c小鼠作為供體，C57BL/6小鼠作為受體，建立背-背皮膚移植模型。術后分為假手術組、模型組、姜黃素（50 mg/kg）組、環(huán)孢素A （10 mg/kg）組、姜黃素+環(huán)孢素A （50 mg/kg+5 mg/kg）組，每組10只，每天1次，ip給藥10 d。記錄各只小鼠移植皮片的存活天數(shù)，術后第4、8天采用ELISA法檢測血漿白細胞介素-2（IL-2）濃度。結果 姜黃素組、環(huán)孢素A組、姜黃素+環(huán)孢素A組的小鼠移植皮片存活時間分別為14.77、16.81、19.96 d，與模型組（12.10 d）比較差異顯著（P<0.01）；姜黃素+環(huán)孢素A組的小鼠移植皮片存活時間與姜黃素或環(huán)孢素A組比較顯著延長（P<0.05、0.01）；姜黃素組、環(huán)孢素A組、姜黃素+環(huán)孢素A組術后第4天血漿IL-2水平分別為3.68、2.05、2.70 ng/mL，與模型組（4.76 ng/mL）比較差異顯著（P<0.05、0.01）；姜黃素組、環(huán)孢素A組、姜黃素+環(huán)孢素A組術后第8天血漿IL-2水平分別為4.06、2.11、2.95 ng/mL，與模型組（5.85 ng/mL）比較差異顯著（P<0.01）。結論 姜黃素具有抑制皮膚移植小鼠免疫排斥反應的作用。

Objective To investigate the immunosuppressive effect of curcumin on xenogenic skin transplantation in mice. Methods The skin transplantation model was established by an operation, tergal skin flaps from BALB/c donor mice transplanted to the back of C57BL/6 recipient mice. Then recipient mice were divided into five groups at random, namely sham, model, curcumin (50 mg/kg), Cyclosporin A (10 mg/kg) and curcumin+Cyclosporin A (50 mg/kg+5 mg/kg). All mice were ip administered once daily for 10 d. The survival days of skin graft were recorded in all groups. The interleukin-2 (IL-2) levels in plasma of all mice were determined by ELISA 4 and 8 d after the operation, respectively. Results The mean survival time of skin graft in curcumin, Cyclosporin A and curcumin+Cyclosporin A groups were 14.77, 16.81 and 19.96 d, respectively, which showed significant differences comparing with 12.10 d of model group (P<0.01). Combination of curcumin and Cyclosporin A administration showed a longer mean survival days than curcumin or Cyclosporin A group (P<0.05 or 0.01). The IL-2 levels in plasma of mice in curcumin, Cyclosporin A and curcumin+Cyclosporin A groups on postoperative day 4 were 3.68, 2.05 and 2.70 ng/mL, respectively, which were significantly reduced than 4.76 ng/mL of model group (P<0.05 or 0.01). The IL-2 levels in plasma of mice in curcumin, Cyclosporin A and combination of curcumin and Cyclosporin A groups on postoperative day 8 were 4.06, 2.11 and 2.95 ng/mL, respectively, which were significantly reduced than 5.85 ng/mL of model group (P<0.01). Conclusion Curcumin may have a good immunosuppressive effect on mice with xenogenic skin transplantation.