目的 研究異煙肼對大鼠淋巴細胞因子表達和巨噬細胞功能的影響。方法 健康雄性SD大鼠，隨機分為喂藥1個月組、喂藥3個月組、停藥1個月（喂藥3個月后）組，每組再隨機等分為異煙肼組和對照組。異煙肼組給以120 mg/kg異煙肼，隔日1次ig給藥，對照組給予生理鹽水。于相應(yīng)時間點，ELISA法檢測血清白細胞介素-12（IL-12）、干擾素-γ（IFN-γ）水平；瓊脂平板法檢測大鼠血清溶菌酶含量；硝酸鉛（Comori）法檢測腹腔液酸性磷酸酶水平。結(jié)果 在各時間點，異煙肼組IL-12、IFN-γ、溶菌酶水平與對照組比較，差異均不顯著；喂藥1個月，與對照組比較，異煙肼組酸性磷酸酶水平顯著升高（P<0.05）；喂藥3個月、停藥1個月，兩組酸性磷酸酶水平差異不顯著。結(jié)論 120 mg/kg異煙肼對大鼠免疫功能無明顯影響，長期應(yīng)用不會導(dǎo)致免疫功能的損傷。

Objective To study the influence of isoniazid on lymphocyte factor expression and macrophage function of rats. Methods Healthy male SD rats were randomly divided into three groups, which was treated for one month, three months and withdrawal for one month after treated for three months, and each group was randomly divided into isoniazid group and control group. The isoniazid groups were ig with isoniazid at dose of 120 mg/kg every other day and control groups were fed on normal saline. At the corresponding time points, the level of interleukin-12 (IL-12) and interferon-γ (IFN-γ) was detected with ELISA method, detected serum lysozyme content by agar plate method, and Comori method was used for the detection of acid phosphatase levels in peritoneal fluid. Results At all the time points, levels of IL-12, IFN-γ and lysozyme in isoniazid group were not significantly different compared with control group. There were statistically significant differences in acid phosphatase between isoniazid group and control group after treated for one month (P<0.05), but the significant differences disappeared at the next two time points. Conclusion Isoniazid of 120 mg/kg may have no obvious influence on the immune function of rat. We don't detect the immune injury.

抗結(jié)核藥物對免疫功能影響的研究（四川省衛(wèi)計委，編號090209）