目的 建立毛郁金藥材中不同成分同時(shí)測定的一測多評法。方法 以毛郁金中莪術(shù)二酮為指標(biāo)，建立莪術(shù)二醇、ocathydro-1，4-dihydroxy-1，4-dimethyl-7-（propan-2-ylidene）azulen-5（1H）-one、原莪術(shù)醇和姜黃素的相對校正因子，在考察相對校正因子的重現(xiàn)性后，進(jìn)一步采用外標(biāo)法對一測多評法所計(jì)算出的成分含量進(jìn)行驗(yàn)證。結(jié)果 10批毛郁金藥材中，各成分采用相對因子計(jì)算的含量與采用外標(biāo)法計(jì)算的結(jié)果無顯著差異。結(jié)論 以莪術(shù)二酮為參照物，采用一測多評測定和計(jì)算其他4種成分含量的方法是可行的，結(jié)果較為準(zhǔn)確。

Objective To develop a method of quantitative analysis of multi-components by single marker (QAMS) for simultaneously determining five compounds in Curcumae Aromaticae Radix. Methods An HPLC method was developed as QAMS to determine curcuma diol, ocathydro-1,4-dihydroxy-1,4-dimethyl-7-(propan-2-ylidene)azulen-5(1H)-one, original curcumol and curcumin in Curcumae Aromaticae Radix, using curdione as intermal reference substance, and the relative correction factor (RCF) of the four components was determined by HPLC with good reproducibility. Their contents in 10 batches of samples, collected from different areas, were determined by both external standard method and QAMS. Result No significant differences were found in the quantitative results of four compounds in 10 batches of Curcumae Aromaticae Radix determined by external standard method and QAMS. Conclusion It is feasible and suitable to evaluate the quality of Curcumae Aromaticae Radix by QAMS.

國家新藥創(chuàng)制項(xiàng)目（2014ZX09101-021-001）