目的 建立小鼠1型糖尿病模型，研究糖尿病早期時睪丸中睪酮合成關鍵酶的變化。方法 雄性成年鼠20只，隨機分為對照組10只、糖尿病模型組10只。通過ip鏈脲佐菌素（150 mg/kg）制備糖尿病模型，成功制備糖尿病模型后4周處死小鼠，取血清和睪丸組織備用。實時熒光定量PCR（qRT-PCR）法檢測睪丸組織中促黃體生成素受體（LHR）、類固醇激素合成靈敏調節(jié)蛋白（StAR）、細胞色素P450膽固醇側鏈裂解酶（P450scc）、3β-羥基類固醇脫氫酶VI型（3β-HSD6）、17a-羥基化酶/17，20-裂解酶I型（P450c17a1）及17β-羥基類固醇脫氫酶Ⅲ型（17β-HSD3）相應mRNA（Lhcgr、Star、Cyp11a1、Hsd3b6、Cyp17a1和Hsd17b3）含量，酶聯免疫吸附法測定血清中睪酮和黃體生成素（LH）含量、睪丸組織中3β-HSD1、P450c17a1及17β-HSD3酶活性。結果 與對照組比較，4周糖尿病小鼠血清中睪酮和LH含量顯著降低（P<0.05）；睪丸組織的Lhcgr、Star、Cyp11a1、Hsd3b6、Cyp17a1和Hsd17b3 mRNA含量顯著降低（P<0.05、0.001）；3β-HSD1、P450c17及17β-HSD3酶活性顯著降低（P<0.05、0.01）。結論 早期糖尿病小鼠睪丸中睪酮合成關鍵酶的表達顯著降低。

Objective Type 1 diabetes mice model was established to investigate the changes of key enzymes involved in testosterone synthesis in testes of early diabetic mice. Methods Tatolly 20 male C57 mice were randomly divided into two groups:control and diabetic groups, and the diabetes mice were ip administered with a single dose of 150 mg/kg Streptozotocin. Four weeks after confirmation of diabetic model, the serum and testis were collected for further study. The qRT-PCR method was used to measure the expression of LHR and steroidogenesis synthetase StAR, P450scc, 3β-HSD6, P450c17a1, and 17β-HSD3 mRNA. ELISA assay was performed to measure the levels of testosterone and luteinizing hormone (LH) in serum, and the enzymatic activities of 3β-HSD1, 1P450c17 and 17β-HSD3 in testis tissue. Results Compared to control group, the levels of testosterone and LH of diabetic group declined significantly (P<0.05) after four weeks. The mRNA levels of LHR, StAR, CYP11a1, Hsd3b6, CYP17a1 and Hsd17b3, and enzymatic activities of 3β-HSD6, P450c17 and 17β-HSD3 were also decreased significantly compared with control group (P<0.05, 0.01 and 0.001). Conclusion Expression of key enzymes of testosterone synthesis in testis of early diabetic mice decreases significantly.

黑龍江省自然科學基金項目（LC201440）；黑龍江省普通本科高等學校青年創(chuàng)新人才培養(yǎng)計劃項目（UNPYSCT-2015110）；黑龍江省大學生創(chuàng)新創(chuàng)業(yè)訓練計劃項目（201410229019）；牡丹江市科學技術計劃項目（Z2015s0038）