目的 觀察川芎嗪對高糖誘導(dǎo)的人視網(wǎng)膜血管內(nèi)皮細胞（HRCECs）增殖的影響。方法 將HRCECs細胞分為對照（生理鹽水）組、模型（25 mmol/L葡萄糖）組和川芎嗪低、中、高濃度（50、100、200 μmol/L）組，培養(yǎng)48 h后，MTT細胞毒實驗檢測細胞增殖變化，流式細胞技術(shù)檢測細胞周期，ELISA法檢測血管內(nèi)皮生長因子（VEGF）表達。結(jié)果 與對照組比較，高糖對HRCECs細胞增殖、分裂（M）期比例、上清VEGF濃度均具有顯著促進作用（P<0.05、0.01）；與模型組比較，低、中、高濃度川芎嗪對高糖誘導(dǎo)的HRCECs細胞增殖、分裂（M）期比例、上清VEGF濃度均發(fā)揮顯著抑制作用（P<0.05、0.01），且呈濃度相關(guān)性。結(jié)論 川芎嗪可能通過抑制高糖誘導(dǎo)的HRCECs細胞VEGF高表達，阻滯細胞周期，發(fā)揮抑制HRCECs細胞增殖的作用。

Objective To observe the effects of Ligustrazine on proliferation of human retinal vascular endothelial cells (HRCECs) induced by high glucose. Methods HRCECs cells were divided into control (saline) group, model (25 mmol/L glucose) group, ligustrazine low, medium, high concentration (50, 100 and 200 mol/L) group, cultured for 48 h. Cell proliferation was detected by MTT cytotoxicity assay. Flow cytometry was used to detect cell cycle, and the expression of VEGF was detected by ELISA analysis. Results Compared with control group, HRCECs cell proliferation, cell division (M) phase ratio and supernatant of VEGF concentration in model group increased significantly (P<0.05, 0.01). Compared with model group, cell proliferation, M phase ratio and supernatant of VEGF in Ligustrazine low, medium and high concentration groups decreased significantly (P<0.05, 0.01), and showed a concentration dependence. Conclusion Ligustrazine can inhibit the expression of VEGF in HRCECs cells induced by high glucose to block cell cycle and inhibit the proliferation of HRCECs cells.