[關(guān)鍵詞]
[摘要]
目的 研究刺老苞根皮水提物對成骨細(xì)胞TGF-β/BMPs信號通路的影響。方法 建立大鼠骨折模型,隨機(jī)分為模型組,刺老苞根皮水提物低、中、高劑量(3.6、1.8、0.9 g/kg)組,ig給藥7 d后腹主動(dòng)脈取血,分離血清;培養(yǎng)大鼠原代成骨細(xì)胞,經(jīng)堿性磷酸酶染色、茜素紅染色鑒定合格后,分別以對應(yīng)組別的動(dòng)物血清連續(xù)培養(yǎng)48 h,采用實(shí)時(shí)熒光定量PCR(qRT-PCR)和Western blotting技術(shù)分別檢測各組細(xì)胞中骨形態(tài)發(fā)生蛋白質(zhì)-2(BMP-2)、轉(zhuǎn)化生長因子-β(TGF-β)、Smad家族相關(guān)蛋白1、2(Smad-1、Smad-2)mRNA和蛋白表達(dá)。結(jié)果 與模型組比較,刺老苞根皮水提物不同濃度的含藥血清組BMP-2、TGF-β、Smad-1 mRNA表達(dá)均顯著升高(P<0.05、0.01),中、高劑量含藥血清組Smad-2 mRNA表達(dá)顯著升高(P<0.05);低、中、高劑量含藥血清組的BMP-2、TGF-β、Smad-1、Smad-2蛋白表達(dá)均顯著升高(P<0.05)。結(jié)論 刺老苞根皮水提物通過上調(diào)TGF-β/BMPs信號傳導(dǎo)通路中的BMP-2、TGF-β、Smad-1、Smad-2表達(dá),促進(jìn)成骨細(xì)胞增殖。
[Key word]
[Abstract]
Objective To study the effect of aqueous extract from Araliae Echinocaulis Radicis et Cortex (AEAE) on expression of TGF-β/BMPs signaling pathway in osteoblasts. Methods The fracture model of rat was established and randomly divided into model group, AEAE low, middle and high dose (3.6, 1.8, 0.9 g/kg) group. After ig administration for 7 d, the blood was taken from abdominal aorta and the serum was separated. The rats primary osteoblasts were cultured and qualified after alkaline phosphatase staining and alizarin red staining. The osteoblasts were cultured continuously for 48 h in animal serum of the corresponding group. The contents of BMP-2, TGF-β, Smad-1 and Smad-2 were detected by qRT-PCR and Western blotting method. Results Compared with model group, the mRNA level of BMP-2, TGF-β and Smad-1 in AEAE low, middle and high dose group, Smad-2 mRNA level in middle and high dose group, and the protein level of BMP-2, TGF-β, Smad-1 and Smad-2 in low, middle and high dose groupwere significantly higher than those in model group(P<0.05 and 0.01). Conclusion AEAE can promote the proliferation of osteoblasts by up-regulating the expression of BMP-2, TGF-β, Smad-1 and Smad-2 in TGF-β/BMPs signaling pathway.
[中圖分類號]
[基金項(xiàng)目]
國家自然科學(xué)基金(81173361、81270051、81473451)