目的 研究淫羊藿水提后藥渣對(duì)去勢(shì)雄性大鼠的壯骨作用。方法 雄性SD大鼠去睪丸造成激素缺乏型骨質(zhì)疏松模型，造模12周后，連續(xù)ig給予淫羊藿水提物及藥渣8周，給藥濃度為0.5、1.0 g/kg（以生藥計(jì)）。給藥結(jié)束后處死動(dòng)物并進(jìn)行血清生化和骨微結(jié)構(gòu)檢測(cè)。結(jié)果 給藥8周，淫羊藿藥渣0.5 g/kg組血清I型前膠原N端前肽（P1NP）含量較模型組明顯升高（P<0.05），發(fā)揮促進(jìn)骨形成作用；血清尿素氮（BUN）、肌酐（Crea）、N-乙酰-β-D-葡萄糖苷酶（NAG）、天冬氨酸轉(zhuǎn)氨酶（AST）、丙氨酸轉(zhuǎn)氨酶（ALT）、總膽固醇（TC）、三酰甘油（TG）、總蛋白（TP）、白蛋白（ALB）、血糖（GLU）水平均呈降低趨勢(shì)，肝腎毒性不明顯；CT掃描結(jié)果顯示，與模型組比較，淫羊藿藥渣0.5 g/kg組脛骨直徑顯著升高（P<0.05）；水提物0.5 g/kg、藥渣1.0 g/kg組骨總體積（TV）顯著升高（P<0.05），各給藥組骨小梁的體積（BV）均呈升高趨勢(shì)，骨表面積（BS）、骨小梁數(shù)量（Tb.N）均顯著升高（P<0.05、0.01）；藥渣1.0 g/kg組骨小梁分離度（Tb.Sp）顯著降低（P<0.01）。結(jié)論 0.5和1.0 g/kg淫羊藿藥渣對(duì)去勢(shì)誘導(dǎo)的雄性大鼠骨質(zhì)疏松有治療作用，效果不低于水提物且沒(méi)有顯著毒性。

Objective To study toxicity and therapeutic effect of the rest of epimedium aqueous extract on osteoporosis in orchidectomized male rats. Methods Male SD rats were orchidectomized to establish osteoporosis model. After 12 weeks of modeling, the aqueous extract of epimedium and the residue were given by ig for 8 weeks continuously, and the concentration was 0.5 and 1.0 g/kg. Then, animals were sacrificed and performed serum biochemical and bone microstructural tests. Results After 8 weeks of administration, the level of serum I type procollagen N terminal propeptide (P1NP) in 0.5 g/kg epimedium residue group was significantly higher than that of model group (P < 0.05), which played an important role in promoting bone formation. The biochemical results showed that the levels of serum urea nitrogen (BUN), creatinine (Crea), N-acetyl-beta-D-glucosamidase (NAG), aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (TC), triacylglycerols (TG), total protein (TP), albumin (ALB), and blood glucose (GLU) were in a decreasing trend and the toxicity of liver and kidney was not obvious. Compared with the model group, CT scanning results showed that the diameter of tibia increased significantly in 0.5 g/kg epimedium residue group (P<0.05); Total volumne of 0.5 g/kg aqueous extract and 1.0 g/kg epimedium residue group was increased significantly (P<0.05); In each group, the trabecular bone volume (BV), bone surface (BS) area and trabecular number (Tb.N) were significantly increased (P<0.05, 0.01); The separation degree of trabecular bone (Tb.Sp) was significantly decreased in 1.0 g/kg epimedium residue group (P<0.01). Conclusion The epimedium residue (0.5 and 1 g/kg) have a significant therapeutic effect on osteoporosis male rats, the effect has no significant difference from aqueous extract of epimedium, and there are no significant toxicity.

國(guó)家重大新藥創(chuàng)制專項(xiàng)課題（2011ZX09201-201-21；2014ZX09304307-001-005）