目的 研究血清胰島素樣生長(zhǎng)因子-1（IGF-1）水平在2型糖尿病（T2D）、帕金森病（PD）及T2D伴發(fā)PD（T2D-PD）患者之間的差異并探討其轉(zhuǎn)錄后調(diào)控機(jī)制。方法 收集T2D患者21例、PD患者20例、T2D-PD患者17例以及正常對(duì)照25例，應(yīng)用ELISA法檢測(cè)血清IGF-1蛋白含量；實(shí)時(shí)熒光定量PCR（qRT-PCR）技術(shù)檢測(cè)血清相關(guān)microRNAs的表達(dá)水平；miRanda分析軟件預(yù)測(cè)miR-206可能靶向IGF-1 mRNA表達(dá)的作用位點(diǎn)。結(jié)果 T2D-PD患者血清IGF-1水平較PD組下降，但差異不顯著，與正常對(duì)照組比較差異不顯著，而較T2D組顯著升高（P<0.001）；T2D-PD組血清miR-206相對(duì)含量顯著低于T2D組（P<0.01），與正常對(duì)照組和PD組比較無顯著性差異；miRanda軟件預(yù)測(cè)miR-206可以靶向性結(jié)合IGF-1 mRNA的3'-非編碼區(qū)（3'-UTR），mirSVR評(píng)分為-1.285，PhastCons評(píng)分為0.6561。結(jié)論 miR-206介導(dǎo)的IGF-1表達(dá)增加可能是糖尿病伴發(fā)PD重要的病理生理機(jī)制，提示血清IGF-1水平可能成為糖尿病伴發(fā)PD早期臨床診斷的關(guān)鍵指標(biāo)之一。

Objective To investigate the difference of serum insulin-like growth factor -1 (IGF-1) levels among patients with type 2 diabetes mellitus (T2D), Parkinson's disease (PD) and type 2 diabetes mellitus with Parkinson's disease (T2D-PD), and to explore the potential mechanism. Methods Totally 21 patients with T2D, 20 patients with PD, T2D-PD in 17 cases and 25 cases of normal control were collected. Serum IGF-1 contents were detected by ELISA and microRNAs expression was measured by qRT-PCR. Moreover, miRanda software was used to predict possible microRNAs in post-transcriptional regulation of IGF-1 mRNA. Results The concentration of IGF-1 in T2D-PD group was significantly higher than that in T2D group (P < 0.001), and there was no significant difference compared with that in normal control or PD patients; The relative content of serum miR-206 in T2D-PD was significantly lower than that in group T2D (P < 0.01), and there was no significant difference compared with that in normal control or PD patients; miRanda software predicted that miR-206 could target the 3'-UTR of IGF-1 mRNA, mirSVR score was -1.285, and PhastCons evaluation was 0.6561. Conclusion miR-206-mediated increase of IGF-1 expression may play an important role in the pathophysiology of diabetes associated with PD, suggesting that serum IGF-1 levels may be one of the key indicators for clinical diagnosis of diabetes mellitus with PD in early stage.

南京醫(yī)科大學(xué)科技發(fā)展基金面上項(xiàng)目（2013NJMU088）；南京醫(yī)科大學(xué)教育研究課題（YB2017032）；大學(xué)生創(chuàng)新創(chuàng)業(yè)計(jì)劃項(xiàng)目（201510312025Z）