目的 研究菟絲子黃酮對(duì)雷公藤多苷片致大鼠睪丸生精細(xì)胞周期阻滯、凋亡及相關(guān)蛋白表達(dá)降低的影響。方法 SD雄性大鼠隨機(jī)分為5組：對(duì)照組，雷公藤多苷片高、低劑量（12、9 mg/kg）組，雷公藤多苷片高、低劑量（12、9 mg/kg）+菟絲子黃酮（生藥100 mg/kg）組，每組6只。每天ig給藥1次，連續(xù)給藥7 d，對(duì)照組大鼠ig給予等體積0.5% CMC-Na溶液；腹主動(dòng)脈采血，制備含藥血清。通過體外培養(yǎng)大鼠睪丸生精細(xì)胞，給予含藥血清干預(yù)24 h，采用流式細(xì)胞儀檢測(cè)生精細(xì)胞周期及凋亡率；采用Western blotting技術(shù)檢測(cè)生精細(xì)胞中SCF/c-kit、C-myc和CREM蛋白表達(dá)。結(jié)果 與對(duì)照組比較，雷公藤多苷片高劑量組使G₀/G₁期生精細(xì)胞百分率顯著降低、S期細(xì)胞百分率顯著升高（P < 0.01），低、高劑量組均可顯著升高生精細(xì)胞凋亡率（P < 0.01）；與等劑量雷公藤多苷片單給藥組比較，配伍菟絲子黃酮后，顯著降低S期細(xì)胞百分率、生精細(xì)胞凋亡率（P < 0.01）。雷公藤多苷片低、高劑量組均使生精細(xì)胞中SCF/c-kit、C-myc和CREM蛋白表達(dá)顯著降低（P < 0.01）；配伍菟絲子黃酮后顯著增加蛋白表達(dá)量（P < 0.01）。結(jié)論 通過合理配伍菟絲子黃酮可顯著改善雷公藤多苷片對(duì)生精細(xì)胞的損傷作用。

Objective To investigate the influence and action mechanism of flavonoids from Cuscutae Semen (FCS) on the cell cycle, apoptosis and protein expression of spermatogenic cells induced by multi-glycoside from Tripterygium Wilfordii (GTW). Methods Male SD rats were randomly divided into five groups:control group, GTW high and low dose (12 and 9 mg/kg) group, high and low dose (12, 9 mg/kg) of GTW + FCS (drug 100 mg/kg) group, 6 rats in each group. Drugs was given once a day for 7 d, and control group was given an equal volume of 0.5% CMC-Na solution. The blood was collected from the abdominal aorta in rats of all the groups, and the serum containing the medicine was prepared. Spermatogenic cells of rats were cultured in vitro. The influence of serum of GTW and GTW + FCS on cell cycle and apoptosis of spermatogenic cells were detected by flow cytometry; and the protein expression of SCF/c-kit, C-myc and CREW in spermatogenic cells induced by serum of GTW and GTW + FCS were detected by western blotting method. Results The percentage of spermatogenic cells in G₀/G₁ phases could be obviously decreased by GTW (12 mg/kg), the percentage of spermatogenic cells in S phase could be obviously increased by GTW (12 mg/kg), and apoptotic rate of spermatogenic cells could be significantly increased by GTW (12 mg/kg and 9 mg/kg, P < 0.01). After compatibility with FCS, the inhibitory effect of cell cycle and apoptosis of spermatogenic cells in GTW group could be significantly improved (P < 0.01). The protein expression of SCF/c-kit, C-myc and CREM could be significantly decreased by GTW 12 and 9 mg/kg (P < 0.01). After compatibility with FCS, the inhibitory effect of protein expression amount of spermatogenic cells in GTW group could be significantly improved (P < 0.01). Conclusion It indicated that when GTW and its related preparations were used in clinic, the related damage of spermatogenic cells induced by GTW and its related preparations could be improved by reasonable compatibility with FCS.

國家自然科學(xué)基金資助項(xiàng)目（81273802）；河南省科技攻關(guān)計(jì)劃項(xiàng)目（132102310245）