目的 研究去氫駱駝蓬堿衍生物B-9-3與5種不同傳統(tǒng)抗腫瘤藥聯(lián)用的體外抑瘤作用。方法 經(jīng)B-9-3（0.78~200.00 μg/mL）與紫杉醇、長春新堿、5-氟尿嘧啶、順鉑和表柔比星（0.78~200.00 μg/mL）單獨(dú)及聯(lián)合作用于肺癌細(xì)胞LLC、結(jié)腸癌細(xì)胞HT-29、肝癌細(xì)胞HepG2和乳腺癌MCF-7 48 h后，采用MTT法檢測腫瘤細(xì)胞存活率，并按照WEBB法計(jì)算聯(lián)合用藥協(xié)同系數(shù)（Q）；進(jìn)一步通過PI/Hoechst 33342雙熒光染色和細(xì)胞劃痕實(shí)驗(yàn)研究B-9-3（0.78、25.00和200.00 μg/mL）作用于LLC細(xì)胞24 h后對瘤細(xì)胞活性、遷移的影響。結(jié)果 與B-9-3單獨(dú)用藥比較，同濃度B-9-3與長春新堿和紫杉醇聯(lián)合用藥，能夠顯著降低MCF-7和HT-29細(xì)胞的存活率，Q均低于0.3（Q < 1為協(xié)同）。熒光染色和細(xì)胞劃痕實(shí)驗(yàn)結(jié)果表明，與溶劑對照組比較，B-9-3（25和200 μg/mL）組細(xì)胞凋亡明顯增多、劃痕修復(fù)率顯著降低（P < 0.01）。結(jié)論 B-9-3顯著抑制LLC細(xì)胞遷移能力并提高細(xì)胞凋亡率，與不同抗腫瘤藥物聯(lián)合具有協(xié)同作用。

Objective To investigate the inhibitory effect of B-9-3 combined with five anti-cancer drugs on different tumor cells. Methods LLC, HT-29, HepG2, and MCF-7 cells were treated with B-9-3 (0.78~200.00 μg/mL) and Vincristine, Taxol, 5-fluorouracil, Cisplatin and Epirubicin in single-group or in combination for 48 h separately. The inhibitory effect on growth of tumor cells was determined by MTT assay, and the combination index (Q) was calculated according to WEBB method. In addition, the apoptosis rate and migration of LLC cells after 24 h treatment of B-9-3 in different consetration (0.78、25.00和200.00μg/mL) were determined by PI/Hoechst33342 fluorescence staining and cell scratch test. Results Compared with B-9-3 alone group, B-9-3 in the same concentration combined with vincristine and paclitaxel reduced the survival rate of MCF-7 and HT-29 cells significantly, and Q was less than 0.3 (Q <1 for synergy). The results of fluorescence staining and cell scratch test showed that, compared with the solvent control group, the apoptosis rate of B-9-3 (25 and 200 g/mL) group increased significantly, and the rate of scratch repairing decreased significantly (P <0.01). Conclusion B-9-3 inhibits the migration of LLC cells and increases the rate of apoptosis significantly, and has synergistic effect with the combination of different antitumor drugs.

兵團(tuán)中青年科技創(chuàng)新領(lǐng)軍人才計(jì)劃（2015BC001）