19F-quantitative nuclear magnetic resonance (qNMR) method using non-deuterated solvent. Methods Using sodium fluoride as an internal standard, emtricitabine as sample and de-ionized water as solvent, the 19F-qNMR of emtricitabine were analyzed. Linear relation test, precision test, stability test and durability test were carried out. Five samples were prepared in parallel for determination, the content of emtricitabine was calculated through response signal area ratios of sample and internal standard. Meanwhile, the mass balance method was used to determine the content of emtricitabine and the two methods were compared. Results In the non-deuterated solvent NMR spectra, NaF and emtricitabine response signal are far away from each other, and there was no interference between them. The method has a good linear relationship, and its precision, stability and durability all meet the requirements. The content of emtricitabine was calculated to be 100.1%, RSD was 0.57% in 19F-qNMR method. The results were consistent with assay calculated by mass balance method (99.7%). Conclusion The 19F-qNMR established in this paper does not require deuterated solvent and provides a effective way to reduce the test cost and environmental pollution."/> 19F-qNMR;mass balance method;determination of content;sodium fluoride"/>