目的 研究西黃丸抑制荷4T1小鼠乳腺癌腫瘤的生長及其可能機制。方法 建立荷4T1小鼠乳腺癌模型，以低、中、高劑量（0.39、0.78、1.95 g/kg） ig給予西黃丸兩周，分離腫瘤組織，稱質量并計算抑瘤率；切片，TUNEL染色檢測腫瘤細胞凋亡；實時熒光定量PCR （qRT-PCR）檢測腫瘤組織JNK1、AP-1 mRNA表達；免疫熒光染色和Western Blotting檢測腫瘤組織中JNK1、AP-1蛋白表達。結果 與模型組比較，腫瘤質量隨西黃丸劑量的升高顯著下降；TUNEL熒光染色結果顯示，腫瘤細胞凋亡數(shù)量隨西黃丸劑量的升高顯著升高；qRT-PCR結果顯示，腫瘤組織中JNK1、AP-1 mRNA表達水平隨西黃丸劑量的升高顯著上調；免疫熒光染色和Western Blotting結果顯示，腫瘤組織中JNK1、AP-1蛋白表達隨西黃丸劑量的升高顯著增加；且高、中、低劑量組差異均具有統(tǒng)計學意義（P<0.05）。結論 西黃丸顯著抑制腫瘤生長，其作用機制與激活JNK1/AP-1信號通路促進腫瘤細胞凋亡相關。

Objective To study the mechanism of Xihuang Pills on growth inhibition of 4T1 breast cancer cells in mice.Methods The model of 4T1 breast cancer mice was established. After administrating Xihuang Pills at low-, medium-, and high-dose level (0.39, 0.78, and 1.95 g/kg) for two weeks, tumor tissue was weighed, sliced, and homogenized. Tumor cell apoptosis was detected by TUNEL staining. The expression of mRNAs in JNK1 and AP-1 in tumor tissue was detected by Real-time quantitative PCR. The expression of JNK1 and AP-1 protein in tumor tissue was detected by immunofluorescence staining and Western Blotting.Results Comparing with model group, the tumor weights of Xihuang Pills group decreased significantly with the increase of doses. TUNEL staining results showed that the number of apoptosis of tumor cells increased with the increase of doses of Xihuang Pills. Real-time PCR results showed that the expression of JNK1 and AP-1 mRNA in tumor tissue increased with the increase of doses of Xihuang Pills. The results of immunofluorescence staining and Western Blotting showed that the expression of JNK1 and AP-1 protein in tumor tissue increased with the increase of doses of Xihuang Pills. The differences in the high, medium and low dose groups were statistically significant (P< 0.05).Conclusion Xihuang Pills significantly inhibit the growth of tumor cells, which mechanism is related to the activation of JNK1/AP-1 signaling pathway, with view to promoting the apoptosis of tumor cells.

國家自然科學基金資助項目（81573664）