目的 探究當(dāng)歸芍藥散防治阿爾茨海默病的作用機(jī)制。方法 選取當(dāng)歸芍藥散中的當(dāng)歸、白芍、白術(shù)、川芎、茯苓、澤瀉為研究對(duì)象，應(yīng)用TCMSP數(shù)據(jù)庫(kù)對(duì)6味中藥主要化學(xué)成分進(jìn)行篩選；使用TCMSP數(shù)據(jù)庫(kù)，對(duì)篩選出的化合物及阿爾茨海默病進(jìn)行靶點(diǎn)預(yù)測(cè)，選取當(dāng)歸芍藥散與阿茨海默病一致的6個(gè)靶點(diǎn)蛋白作為后續(xù)研究對(duì)象，通過(guò)生物分子功能注釋系統(tǒng)（MAS 3.0）及KEGG通路數(shù)據(jù)庫(kù)進(jìn)行通路注解及分析，得到當(dāng)歸芍藥散治療阿茨海默病的相關(guān)靶點(diǎn)通路預(yù)測(cè)圖及相關(guān)信號(hào)通路。結(jié)果 從當(dāng)歸芍藥散中篩選出35個(gè)化合物，可作用于阿爾茨海默病6個(gè)潛在的蛋白靶點(diǎn)，找出靶點(diǎn)的相關(guān)通路22條。作用通路涉及阿爾茨海默病發(fā)病機(jī)制相關(guān)的鈣信號(hào)途徑、炎癥、免疫調(diào)節(jié)、細(xì)胞與細(xì)胞之間的信號(hào)交流、肌動(dòng)蛋白細(xì)胞骨架的調(diào)節(jié)等各個(gè)環(huán)節(jié)，6味中藥既有共同的成分及作用靶點(diǎn)、通路，又各有偏重，各通路間通過(guò)共有靶點(diǎn)連接，顯示出不同成分間的多靶點(diǎn)、多途徑的協(xié)同作用。結(jié)論 預(yù)測(cè)出當(dāng)歸芍藥散治療阿爾茨海默病可能與其調(diào)節(jié)炎癥、免疫系統(tǒng)、鈣信號(hào)、細(xì)胞與細(xì)胞之間的信號(hào)交流等機(jī)制有關(guān)。

Objective To explore the mechanism of Danggui-Shaoyao-San(DSS)for the treatment of Alzheimer's disease.Methods Angelicae Sinensis Radix, Paeoniae Radix Alba, Chuanxiong Rhizoma, AtractylodesMacrocephalaKoidz, PoriaCocos, and Alisma Orientale were selected as the research object. TCMSP database was applied to screening the main chemical components of DSS. Forecast the potential targets using TCMSP database for Alzheimer's disease and the main chemical components, and selected six consistent target proteins as a follow-up object. Through the MAS 3 and KEGG pathway database, path annotation and analysis were carried out. Finally, DSS related target pathway for the treatment of Alzheimer's disease prediction map and the related signaling pathways were obtained.Results Six potential protein targets and 22 targets related pathways related with Alzheimer's disease were identified in DSS 35 components, including calcium signaling pathways, inflammation, immune system, signal exchange between cells and cells, the regulation of actin cytoskeleton, and so on. The targets and signaling pathways among the six flavour Chinese drug components, and each have lay particular stress on, through mutual targets for connections between various pathways, shows a different composition between the target and way of synergy.Conclusion Danggui-Shaoyao-San for treatment of Alzheimer's Disease may be linked to regulate inflammation, immune system, calcium signal and signal exchange between cells and cell life activities.

湖南省教育廳開(kāi)放基金項(xiàng)目（15k088）；湖南中醫(yī)藥大學(xué)科技創(chuàng)新團(tuán)隊(duì)項(xiàng)目（201609）