目的 動(dòng)態(tài)觀察組分配伍四逆湯對(duì)甲減大鼠下丘腦-垂體-甲狀腺軸的影響。方法 ig 0.1%丙基硫氧嘧啶（PTU）混懸液法分別造模6、11、16、20周，用左甲狀腺素鈉片（9 μg/kg）及組分配伍四逆湯（生藥9.6 g/kg）對(duì)模型動(dòng)物治療4周，酶聯(lián)免疫吸附法測(cè)定大鼠血清三碘甲狀腺原氨酸（T₃）、甲狀腺素（T₄）、促甲狀腺激素（TSH）、促甲狀腺素釋放激素（TRH）水平的變化。結(jié)果 甲減程度隨著造模時(shí)間的延長(zhǎng)而加重。造模6、11、16周甲減大鼠與對(duì)照組比較，模型組大鼠血清T₃、T₄、TRH水平顯著降低（P<0.05、0.01），TSH水平顯著升高（P<0.05、0.01）；與模型組比較，左甲狀腺素鈉片組及組分配伍四逆湯組大鼠血清中T₃、T₄、TRH水平均顯著升高（P<0.05、0.01），TSH水平顯著降低（P<0.05、0.01）。造模20周甲減大鼠與對(duì)照組比較，模型組大鼠血清T₃、T₄、TRH水平顯著降低（P<0.01），TSH水平明顯升高（P<0.01），左甲狀腺素鈉片組和組分配伍四逆湯組血清T₃、T₄水平均顯著降低（P<0.05、0.01）；與模型組比較，左甲狀腺素鈉片組及組分配伍四逆湯組大鼠血清T₃、T₄無統(tǒng)計(jì)學(xué)差異，TRH水平顯著升高（P<0.05、0.01），TSH水平顯著降低（P<0.01）。結(jié)論 下丘腦-垂體-甲狀腺軸在甲減發(fā)展過程中整體功能在退化，組分配伍四逆湯的調(diào)節(jié)作用是動(dòng)態(tài)變化的，應(yīng)在初期及早給予。

Objective To investigate the dynamic changes of component compatibility Sini Decoction on hypothalamic-pituitary-thyroid axis in hypothyroidism rats. Methods The hypothyroidism model of 6, 11, 16, and 20 weeks were established by 0.1% propylthiouracil (PTU) solution. Rat were randomly divided into model group, Sini Decoction group (9.6 g/kg; 4 weeks), and positive control group (Euthyrox 9 μg/kg; 4 weeks). ELISA method was used to determine the serum T₃, T₄, TSH, and TRH levels. Results The degree of hypothyroidism increased with the extension of time. Compared with control group, serum T₃, T₄,and TRH levels in model group of 6, 11 and 16 weeks hypothyroidism rats were significantly decreased (P<0.05 or 0.01), while the TSH level was increased (P<0.05 or 0.01). Compared with model group, serum T₃, T₄, and TRH contents were notably increased (P<0.05 or 0.01), and serum TSH content was decreased (P<0.05 or 0.01) in Sini Decoction group and positive control group. Compared with control group, serum T₃, T₄, and TRH levels in model group of 20 weeks were significantly decreased (P<0.01) and the TSH level was increased (P<0.01). However, the serum T₃ and T₄ contents of Sini Decoction group and positive control group were markedly decreased (P<0.05 or 0.01). Compared with model group, there were no statistically significant differences in the serum T₃ and T₄ contents in Sini Decoction group and positive control group, while the TRH contents were notably increased (P<0.05 or 0.01) and TSH level was decreased (P<0.01). Conclusion Total function of hypothalamic-pituitary-thyroid axis degenerated in the process of hypothyroidism, and the regulating effect of component compatibility Sini Decoction dynamically changed, which should be given as soon as possible in the early stage.

國(guó)家自然科學(xué)基金自助項(xiàng)目（81373546）