2)水平變化;光鏡下觀察卵巢形態(tài)與卵泡數(shù);實時熒光定量PCR(qRT-PCR)檢測大鼠卵巢生長分化因子9(GDF-9)、早期生長反應(yīng)因子1(Egr-1)mRNA表達(dá)水平的變化;免疫組化法檢測大鼠卵巢GDF-9、Egr-1蛋白表達(dá)水平的變化。結(jié)果 與模型組比較,坤泰膠囊組大鼠精神逐漸好轉(zhuǎn),飲食恢復(fù)正常,毛色有光澤,大便色深;體質(zhì)量漸增,差異顯著(P<0.05);動情周期紊亂得到明顯改善(P<0.05);卵巢存在成熟卵泡,卵泡腔內(nèi)卵子生長良好;大鼠血清FSH、LH水平明顯降低,E2、AMH水平明顯升高,差異有統(tǒng)計學(xué)意義(P<0.05);GDF-9、Egr-1蛋白及基因表達(dá)明顯增加,差異有統(tǒng)計學(xué)意義(P<0.05),且作用均呈劑量相關(guān)性,高劑量組治療效果與結(jié)合雌激素片無明顯差距。結(jié)論 坤泰膠囊能夠修復(fù)大鼠受損的卵巢組織結(jié)構(gòu),提高成熟卵泡存在數(shù)量,改善大鼠動情周期及激素水平,機制可能與增加GDF-9、Egr-1表達(dá)相關(guān)。;Objective To explore the therapeutic effects and mechanism of Kuntai Capsules on ovarian reserve dysfunction in rats induced by cyclophosphamide injection. Methods The 60 12-week-old SD female rats were randomly divided into control group, model group, Kuntai Capsules high, middle, and low dose (1.2, 0.6, and 0.3 g/kg) groups, and estrogen group. Cyclophosphamide peritoneal injection was used to establish the model of ovarian reserve dysfunction. Rats in each drug group were ig administrated successively for 36 d after 14 d of modeling. During the experiment, the behavior and the changes of body mass of ratswere observed. Regular vaginal smear was done to observe the changes of estrus cycle in rats. Serum sex hormone levels changes of antimullerian hormone (AMH), and follicle-stimulating hormone (FSH), luteinizing hormone (LH), and Estradiol (E2) were detected by enzyme-linked immunosorbent assay (ELISA). The ovary morphology and follicular number were observed under light microscope. Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the changes in mRNA expression levels of growth differentiation factor 9 (GDF-9) and early growth response gene 1 (Egr-1) in rats. The expression levels of GDF-9 and Egr-1 in rats were measured by immunohistochemistry. Results Compared with the model group, the spirit of the rats in the Kuntai Capsules group gradually improved, the diet was restored to normal, the hair color was luster, the color of the stool was deep, the body mass was increasing, the difference was significant (P<0.05); The disorder of the estrous cycle was obviously improved (P<0.05); The ovary had mature follicles and the follicles grew well in the follicle cavity; The level of FSH and LH in the serum of rats decreased significantly, the level of E2 and AMH increased significantly (P<0.05), and the expression of GDF-9, Egr-1 protein and gene increased significantly (P<0.05), and the effect was in a dose-dependent manner. There were no significant therapeutic effects between Kuntai Capsules high dose group and Conjugated Estrogens Tablets group. Conclusion Kuntai Capsules could repair damaged ovarian tissue of rats structure, increase the number of mature follicles, and improve estrous cycle and hormone level in rats, which may be related to the increased expression of GDF-9 and Egr-1."/>

醉酒后少妇被疯狂内射视频,一本色道久久综合一,在线天堂新版资源www在线下载,中文字幕乱人伦高清视频,中字幕视频在线永久在线观看免费

首頁 > 過刊瀏覽>2018年第41卷第5期 >2018,41(5):780-787. DOI:10.7501/j.issn.1674-6376.2018.05.e011
上一篇 | 下一篇

坤泰膠囊對卵巢儲備功能低下大鼠的治療作用及對卵巢GDF-9、Egr-1表達(dá)的影響

Effects of Kuntai Capsules on expression of ovarian GDF-9 and egr-1 in rats with diminished ovarian reserve

發(fā)布日期:2018-05-09
您是第位訪問者
藥物評價研究 ® 2025 版權(quán)所有
技術(shù)支持:北京勤云科技發(fā)展有限公司
津備案:津ICP備13000267號 互聯(lián)網(wǎng)藥品信息服務(wù)資格證書編號:(津)-非經(jīng)營性-2015-0031