目的 觀察還貝止咳方對肺炎鏈球菌致肺熱模型大鼠的胸腺、脾臟指數(shù)，炎性細(xì)胞及炎性因子的影響。方法 SD幼年大鼠隨機分為對照組、模型組、頭孢呋辛酯片組、小兒肺熱咳喘顆粒組及還貝止咳方的低、中、高劑量組，采用鼻腔滴入肺炎鏈球菌的方法建立大鼠肺熱模型，造模前后監(jiān)測大鼠體溫，評價大鼠肺熱模型；采用流式細(xì)胞儀及全血分析儀檢測大鼠全血中的炎性細(xì)胞；采用酶聯(lián)免疫技術(shù)檢測大鼠肺灌洗液（BALF）中的白細(xì)胞介素（IL-4、IL-1β、IL-6、IL-10）、腫瘤壞死因子-α（TNF-α）、血清免疫球蛋白E（IgE）水平；完整剝離大鼠脾臟和胸腺，檢測脾臟指數(shù)和胸腺指數(shù)。結(jié)果 與對照組比較，模型組大鼠的體溫明顯升高（P<0.01），說明造模成功。與模型組比較，還貝止咳方低、中、高劑量組大鼠體溫均顯著降低（P<0.05、0.01），全血中的白細(xì)胞、嗜酸性粒細(xì)胞數(shù)目、Th/Ts比值均顯著降低（P<0.05、0.01），CD3⁺CD4⁺、CD3⁺CD8⁺細(xì)胞比例顯著上升（P<0.05、0.01）；BALF中的IL-10、IL-1β、IL-6、IL-4、TNF-α的水平明顯降低（P<0.05、0.01）；且對IL-1β及TNF-α的下調(diào)作用優(yōu)于陽性對照藥；脾臟指數(shù)明顯升高（P<0.05、0.01）。結(jié)論 還貝止咳方對細(xì)菌性大鼠肺炎模型具有一定的治療作用，主要通過調(diào)節(jié)大鼠的免疫功能，減輕炎性反應(yīng)發(fā)揮作用。

Objective To observe the effect of Huanbei Zhike Recipe on thymus, spleen index, inflammatory cells, and inflammatory cytokines in lung heat model rats induced by Streptococcus pneumoniae. Methods SD young rats were randomly divided into control group, model group, Cefuroxime Axetil Tablets group, children's lung hot cough and asthma granule group and Huanbeizhike recipe lower, middle and high dose group. The rat lung heat model was established by the nasal drip of Streptococcus pneumoniae. The body temperature of the rats was monitored before and after modeling to evaluate the rat model of pulmonary hyperthermia. The flow cytometry and whole blood analyzer were used to detect the inflammatory cells in rat whole blood. The secretion levels of IL-10, IL-1β, IL-6, IL-4, TNF-α and IgE in rat lung lavage fluid (BALF) were detected by enzyme-linked immunosorbent assay. The spleen and thymus were stripped completely, and spleen index and thymus index were detected. Results The body temperature of model group was significantly increased (P<0.01), indicating successful modeling. Compared with the model group, the body temperature, leukocyte, neutrophil number and Th/Ts ratio in the whole blood of Huanbeizhike recipe low, medium and high dose groups were significantly decreased and CD3⁺CD4⁺ and CD3⁺CD8⁺ increased significantly (P<0.05 and 0.01). The levels of IL-10, IL-1β, IL-6, IL-4 and TNF-α of BALF were significantly decreased (P<0.05 and 0.01) of Huanbeizhike recipe, and the down-regulation effect of IL-1β and TNF-α was better than that of positive control. The spleen index of Huanbeizhike recipe was significantly increased (P<0.05 and 0.01). Conclusion Huanbeizhike Recipe has a certain therapeutic effect on the bacterial rat pneumonia model, mainly through the regulation of the immune function of rats to reduce the inflammatory effect.

山西省重點研發(fā)計劃（201603D321028）