目的 建立同時適用于5種丹參制劑（丹參片、復(fù)方丹參片、冠心丹參膠囊、心可舒片、雙丹膠囊）中4個酚酸類成分一測多評的含量測定方法。方法 采用高效液相色譜法，以丹參素為內(nèi)參物，建立原兒茶醛、迷迭香酸、丹酚酸B與丹參素的相對校正因子，比較相對保留值法和保留時間差法對成分定位的影響，并采用外標(biāo)法驗證結(jié)果的可靠性。結(jié)果 采用相對保留值法可對待測成分進行準確定位，所建立的相對校正因子耐用性良好，原兒茶醛、迷迭香酸、丹酚酸B相對于丹參素的相對保留值分別為1.894、5.379、6.599，相對校正因子分別為6.611、3.845、2.059，采用外標(biāo)法驗證結(jié)果無顯著性差異。結(jié)論 該方法穩(wěn)定、可靠，可為上述丹參制劑質(zhì)量控制提供參考。

Objective A simple, rapid and low-cost quantitative analysis method of multi-components by single marker (QAMS) was developed to the determination of danshensu, protocatechuic aldehyde, rosmarinic acid and salvianolic acid B in Danshen preparations (Danshen Tablet, Fufang Danshen Tablet, Guanxin Danshen Capsule, Xin-Ke-Shu Tablet, Shuangdan Capsule). Methods Danshensu was selected as the internal reference standard, and the relative correction factors were calculated between other three components with danshensu. The relative retention value method and the retention time difference method were compared on the component localization. The external standard method was used to verify the reliability of the QAMS results. Results The tesults showed the relative correction factors of protocatechuic aldehyde, rosmarinic acid and salvianolic acid B were 6.611, 3.845, 2.059, respectively. The relative retention times of protocatechuic aldehyde, rosmarinic acid and salvianolic acid B were 0.314, 0.518, and 0.561, respectively, for accurate location. The QAMS method could assure to obtain consistent results with the external standard method. Conclusion This proposed QAMS method could provide an effective strategy for routine quantitative analysis and quality control of Danshen preparations.

國家科技支撐計劃：基于本草基因組的中藥材活性成分生合成及育種示范研究（2012BAI29B01）