目的 建立金蓮花湯中綠原酸、咖啡酸、葛根素、牡荊素、迷迭香酸的HPLC含量測定方法，并對采用不同來源的藥材制備的3批金蓮花湯中的上述5種成分進(jìn)行含量測定。方法 采用HPLC法，色譜柱為Synergi Polar-RP苯基C₁₈色譜柱（250 mm×4.6 mm，4 μm）；流動相為1%乙酸（A）～乙腈（B）；洗脫程序（0～30 min，5%～14% B；30～40 min，14%～14% B；40～42 min，14%～19% B；42～60 min，19%～25% B；60～63 min，25%～5% B；63～64 min，5%～5% B）；柱溫35℃；檢測波長286 nm；進(jìn)樣量10 μL。結(jié)果 測定3批金蓮花湯中綠原酸、咖啡酸、葛根素、牡荊素、迷迭香酸含量分別為：0.34、0.08、9.73、0.93、0.32 mg/g；0.13、0.03、8.37、0.33、0.21 mg/g；0.37、0.07、8.36、0.12、0.26 mg/g。結(jié)論 該方法精密度、穩(wěn)定性、重復(fù)性良好，適用于金蓮花湯中上述5種成分的含量測定；用不同來源的藥材制備的金蓮花湯中上述5種成分的含量存在差異，其中葛根素的含量最高。

Objective To establish an HPLC method for content assay of chlorogenic acid, caffeic acid, puerarin, vitexin and rosmarinic acid in Jinlianhua decoction, and the contents of the above five components were determined in three batches of Jinlianhua decoction prepared from the drugs bought from different places of China. Methods The analysis was performed on a Synergi Polar-RP Phenomenex C₁₈ column (250 mm×4.6 mm, 4 μm) using 1% acetic acid solution (A) - acetonitrile (B) as the mobile phases with gradient elution (0-30 min, 5%-14% B; 30-40 min, 14%-14% B; 40-42 min, 14%-19% B; 42-60 min, 19%~25% B; 60-63 min, 25%-5% B; 63-64 min, 5%-5%B). The column temperature was set at 35℃, the UV detection wavelength was 286 nm, and the injection volume was 10 μL. Results The contents of chlorogenic acid, caffeic acid, puerarin, vitexin and rosmarinic acid in the three batches of Jinlianhua decoction were determined as follows:0.34, 0.08, 9.73, 0.93, 0.32 mg/g; 0.13, 0.03, 8.37, 0.33, 0.21 mg/g; 0.37, 0.07, 8.36, 0.12, 0.26 mg/g. Conclusion The method is precise, stable and reproducible. It is suitable for the determination of chlorogenic acid, caffeic acid, puerarin, vitexin and rosmarinic acid in Jinlianhua decoction. The content of these fives components is different in the Jinlianhua decoctions prepared from the drugs purchased from different places of China, and that of puerarin is the highest.

北京中醫(yī)藥大學(xué)新奧獎勵基金（金蓮花湯的抗流感病毒有效成分群的獲取及其藥效物質(zhì)基礎(chǔ)研究）