目的 研究小分子阿膠對小鼠免疫功能的調(diào)節(jié)作用。方法 將ICR小鼠隨機(jī)分為對照組、模型組、生血丸3.00 g/kg組和小分子阿膠3.00、1.50、0.75 g/kg組，ig給藥14 d，ip環(huán)磷酰胺或氫化可的松建立免疫低下模型，用雞紅細(xì)胞誘導(dǎo)小鼠溶血素，測定小鼠血清溶血素水平，觀察小分子阿膠對體液免疫的影響；采用二硝基氯苯誘導(dǎo)小鼠耳廓腫脹，檢測小鼠耳廓腫脹度，觀察小分子阿膠對細(xì)胞免疫的影響；利用流式細(xì)胞術(shù)對小鼠淋巴細(xì)胞亞群進(jìn)行分析，觀察小分子阿膠對小鼠淋巴細(xì)胞分型的影響。結(jié)果 與模型組比較，小分子阿膠3.00、1.50、0.75 g/kg顯著提高免疫功能低下小鼠血清溶血素含量（P<0.01）；3.00、1.50 g/kg顯著提高小鼠耳廓腫脹度（P<0.01、0.05）；3.00 g/kg顯著提高小鼠T淋巴細(xì)胞（CD3⁺）、輔助性T細(xì)胞和遲發(fā)超敏性T細(xì)胞（CD3⁺CD4⁺）占淋巴細(xì)胞的比例（P<0.01）。結(jié)論 小分子阿膠能提高小鼠體液免疫和細(xì)胞免疫功能，對免疫功能有正向調(diào)節(jié)作用。

Objective To study the effect of small molecular Asini Corii Colla (SMACC) on immune function in mice. Methods ICR mice were randomly divided into control group, model group, Shengxue Pills (3.00 g/kg) group, and SMACC (3.00, 1.50, 0.75 g/kg) group, and they were ig administered for 14 d. Immunosuppressive model was established by intraperitoneal injection of cyclophosphamide or hydrocortisone. The use of red blood cells in chicken was to induce the production of hemolysin in mice. The content of serum hemolysin was measured to determine the effect of SMACC on humoral immunity. The ear swelling of mice was induced by two nitrochlorobenzene, and the auricular swelling of mice was detected to observe the effect of SMACC on cell immunity. Lymphocyte subsets in mice were analyzed by flow cytometry to observe the effect of SMACC on lymphocyte subtyping. Results Compared with model group, SMACC significantly increased the serum hemolysin content at doses of 3.00, 1.50, and 0.75 g/kg (P < 0.01), significantly increased the degree of auricular swelling in mice at doses of 3.00 and 1.50 g/kg (P < 0.01 and 0.05), and significantly promoted the proportion of T lymphocytes (CD3⁺), helper T cells, and delayed hypersensitivity T cells (CD3⁺CD4⁺) at doses of 3.00 g/kg (P < 0.01). Conclusions SMACC can enhance humoral immunity and cellular immune function in mice and has a positive regulatory effect on immune function in mice.

國家科技重大專項(xiàng)（2015ZX09501004）；天津市科技計(jì)劃項(xiàng)目（16PTGCCX00090）；國家中藥標(biāo)準(zhǔn)化項(xiàng)目—阿膠標(biāo)準(zhǔn)化建設(shè)（ZYBZH-Y-SD-31）；泰山產(chǎn)業(yè)領(lǐng)軍人才工程專項(xiàng)經(jīng)費(fèi)資助