目的 建立高效液相色譜法測定田七痛經(jīng)膠囊中阿魏酸、藁本內酯、歐當歸內酯A的方法。方法 選用Thermo Acclaim C₁₈色譜柱，Thermo SCIENTIFICSyncronis C₁₈色譜柱；檢測波長為321、280 nm；體積流量1.0 mL/min；柱溫35℃結果 阿魏酸、藁本內酯、歐當歸內酯A的質量濃度與峰面積分別在1.12～112.30、1.33～132.60、0.96～95.50 μg/mL呈良好的線性關系；回歸方程分別為Y=46 335 540 X+44 186.71（r=0.998），Y=14 514 060 X+8 715.27（r=0.999），Y= 22 031 250 X+16 472.10（r=0.999）。結論 該方法準確，簡便，靈敏，可作為田七痛經(jīng)膠囊質量控制的方法。

Objective Establishing aRP-HPLC method for the determination of the contents of Ferulic acid, Ligustilide and Levistolide A in tianqi tongjing capsule. Methods Thermo Acclaim C₁₈ column,Thermo SCIENTIFIC Syncronis C₁₈ column was used. The detection wavelength was set at 321 nm, 280 nm. The flow rate was 1.0 mL/min. The column temperature was maintained at 35℃. Results Ferulic acid was linear in the range from 1.12 to 112.30 μg/mL and the regression equation was Y=46 335 540 X + 44 186.71 (r=0.998). Ligustilide was linear in the range from 1.33 to 132.60 μg/mL and the regression equation was Y=14 514 060 X + 8 715.27 (r=0.999). Levistolide A was linear in the range from 0.96 to 95.50 μg/mL and the regression equation was Y=22 031 250 X + 16 472.10 (r=0.999). Conclusion The method is accurate, simple and feasible, and can be used as an ideal method for the quality control of tianqi tongjing capsule.

國家評價性抽驗項目