目的 研究銀杏內(nèi)酯注射液（GIs）及其主要組分銀杏內(nèi)酯B（GB）和白果內(nèi)酯（BB）對急性缺血性腦損傷的改善作用，以及GIs對突觸后致密物95（PSD95）蛋白表達的影響。方法 ①采用Longa法制備大鼠大腦中動脈阻塞（tMCAO）模型，于缺血再灌注1 h后給予GIs（2.5、5.0 mg/kg）、GB（1.25、2.50 mg/kg）、BB（1.25、2.50 mg/kg），每天給藥2次，連續(xù)給藥3 d。于術(shù)后24、48、72 h各進行神經(jīng)功能評分1次；TTC染色法測定腦梗死體積；稱大鼠干濕質(zhì)量，測定腦組織含水量。②大鼠隨機分為假手術(shù)組、GIs單給藥（2.5 mg/kg）組、模型組和GIs治療（tMCAO+GIs 2.5 mg/kg）組，制備tMCAO模型，再灌1 h后開始ip給藥，3 d內(nèi)每天給藥2次。分別于再灌注后24、72 h取腦，Western blotting法檢測缺血半影區(qū)PSD 95蛋白表達水平。結(jié)果 ①與模型組比較，GIs及其主要組分GB、BB均顯著改善tMCAO模型大鼠神經(jīng)運動功能障礙，減少腦梗死體積，降低腦水腫（P<0.05、0.01）；同等劑量下（2.5 mg/kg），GIs治療效應優(yōu)于單組份GB、BB。②GIs組給藥后胞漿PSD95蛋白水平均較模型組顯著升高（P<0.05、0.01）。結(jié)論 GIs及其有效組分GB、BB均具有改善tMCAO大鼠急性缺血性損傷的作用，GIs的作用優(yōu)于GB、BB單獨用藥，作用機制可能與上調(diào)PSD95蛋白表達相關。

Objective To study the effects of Ginkgolides Injection (GIs) and its main components Ginkgolides B (GB) and Baiguolide (BB) on acute ischemic brain injury, and the effects of GIs on the expression of postsynaptic dense substance 95 (PSD95). Methods ① A 90 min transcient middle cerebral artery occlusion model (tMCAO) was established using Longa method. Rats were randomly divided into seven groups:tMCAO group, GIs (2.5, 5.0 mg/kg) groups, GB (1.25, 2.5 mg/kg) groups and BB (1.25, 2.5 mg/kg) groups. Rats were ip administered after 1 h of reperfusion, and continuously for three days. Neurological deficit score, infarct size (TTC staining) and brain water content were measured at 72 h after reperfusion. ② Rats were randomly divided into four groups:sham group, sham + GIs groups (2.5 mg/kg), tMCAO groups, and tMCAO + GIs groups (2.5 mg/kg). Rats were ip administered after 1 h of reperfusion, and continuously for three days, twice a day. The PSD 95 protein level in cerebral ischemic penumbra was measured by Western blotting at 24 and 72 h after reperfusion. Results ① Compared with model group, GIs and its main components GB and BB significantly improved neuromotor dysfunction, reduced cerebral infarction volume and brain edema in tMCAO model rats (P<0.05, 0.01); At the same dose (2.5 mg/kg), the effect of GIs treatment was better than that of single component GB and BB. ② The level of PSD95 protein in GIs group was significantly higher than that in model group (P<0.05, 0.01). Conclusion GIs and its active constituents, GB and BB have therapeutical effect in acute ischemic brain injury induced by tMCAO in rats, and GIs was better than single treatment of GB and BB. The mechanism may be related to the up-regulation of PSD95 protein expression.

R962.2