[關(guān)鍵詞]
[摘要]
目的 建立快速、靈敏、高效的精氨酸雙糖苷的測定方法并研究精氨酸雙糖苷在大鼠體內(nèi)的藥動學(xué)。方法 以烏苯美司作為內(nèi)標(biāo),采用LC-MS/MS檢測方法測定血漿中精氨酸雙糖苷濃度,利用DAS2.0軟件計算藥動學(xué)相關(guān)參數(shù),并分析方法的可靠性。結(jié)果 空白血漿對檢測物質(zhì)不產(chǎn)生干擾,在20~20 000 ng/mL符合線性關(guān)系,方法學(xué)中RSD以及RE值均小于15%。結(jié)論 采用LC-MS/MS方法檢測精氨酸雙糖苷方法合理可靠,精氨酸雙糖苷的絕對生物利用度為(4.50±3.75)%。
[Key word]
[Abstract]
Objective To develop a quick, specific and high performance method for determination of Arginyl-fructosyl-glucose (AFG) and to investigate pharmacokinetics of AFG in rats in vivo. Methods Bestatin is used as an internal standard, the concentration of AFG in plasma was determined by LC-MS/MS. The DAS 2.0 software was adopted to calculate bioavailability of AFG. Results Blank plasma does not interfere with the test substance, there is a significant correlation in the range of 20 ng/mL to 20 000 ng/mL, and the RSD and RE values are less than 15% in the method. Conclusion The average relative bioavailability of AFG was (4.50 ±3.75)%, The measured method of AFG by LC-MS/MS is reasonable and reliable.
[中圖分類號]
R969
[基金項(xiàng)目]
遼寧省高等學(xué)校創(chuàng)新團(tuán)隊支持計劃(LT2016017);大連市科技創(chuàng)新基金(2018J12SN062)