目的 建立快速、靈敏、高效的精氨酸雙糖苷的測(cè)定方法并研究精氨酸雙糖苷在大鼠體內(nèi)的藥動(dòng)學(xué)。方法 以烏苯美司作為內(nèi)標(biāo)，采用LC-MS/MS檢測(cè)方法測(cè)定血漿中精氨酸雙糖苷濃度，利用DAS2.0軟件計(jì)算藥動(dòng)學(xué)相關(guān)參數(shù)，并分析方法的可靠性。結(jié)果 空白血漿對(duì)檢測(cè)物質(zhì)不產(chǎn)生干擾，在20~20 000 ng/mL符合線性關(guān)系，方法學(xué)中RSD以及RE值均小于15%。結(jié)論 采用LC-MS/MS方法檢測(cè)精氨酸雙糖苷方法合理可靠，精氨酸雙糖苷的絕對(duì)生物利用度為（4.50±3.75）%。

Objective To develop a quick, specific and high performance method for determination of Arginyl-fructosyl-glucose (AFG) and to investigate pharmacokinetics of AFG in rats in vivo. Methods Bestatin is used as an internal standard, the concentration of AFG in plasma was determined by LC-MS/MS. The DAS 2.0 software was adopted to calculate bioavailability of AFG. Results Blank plasma does not interfere with the test substance, there is a significant correlation in the range of 20 ng/mL to 20 000 ng/mL, and the RSD and RE values are less than 15% in the method. Conclusion The average relative bioavailability of AFG was (4.50 ±3.75)%, The measured method of AFG by LC-MS/MS is reasonable and reliable.

R969

遼寧省高等學(xué)校創(chuàng)新團(tuán)隊(duì)支持計(jì)劃（LT2016017）；大連市科技創(chuàng)新基金（2018J12SN062）