目的 探討天料木中異香豆素糖苷化合物4-hydroxy-2-{[（benzoyl） oxy]methyl}phenyl-β-D-glucopyranoside-6-benzoate （HPGB）對小鼠胚胎成骨細胞MC3T3-E1細胞活性的影響及其作用機制。方法 體外培養(yǎng)MC3T3-1細胞，用不同濃度的異香豆素糖苷化合物進行干預，采用MTT法測定MC3T3-1細胞增殖情況，堿性磷酸酶（ALP）測定細胞分化情況，懸液芯片技術(shù)檢測、分析糖蛋白Dickkop （DKK-1）、骨保護素（OPG）、骨橋蛋白（OPN）、骨鈣素（BGP）和核因子-κB受體活化因子配體（RANKL）蛋白表達水平。Real-time PCR法檢測、分析DKK-1、RANKL mRNA的表達水平。結(jié)果 異香豆素糖苷化合物在3~300 μmol/L可促進MC3T3-E1細胞增殖和分化能力，具有濃度相關(guān)性；與正常對照組相比，異香豆素糖苷化合物可提高MC3T3-E1的ALP活性。蛋白結(jié)果表明，異香豆素糖苷化合物可顯著上調(diào)MC3T3-E1細胞中OPG、OPN的表達，DKK-1、RANKL蛋白表達無明顯變化，但顯著提高了OPG/RANKL值。同時，mRNA結(jié)果顯示異香豆素糖苷化合物可顯著上調(diào)MC3T3-E1細胞中OPG mRNA表達，顯著提高OPG/RANKL值。結(jié)論 異香豆素糖苷化合物可通過上調(diào)OPG、OPN的表達、提高OPG/RANKL值，促進MC3T3-E1的增殖和分化。

Objective To explore the possible mechanism of isocoumarin glycoside from the stems of Homalium paniculiflorum on MC3T3-E1 cells activity.Methods The MC3T3-E1 cells were cultured and stimulated with various concentrations of isocoumarin glycoside for 48 h. And then, MTT and ALP assay were used to analyze the proliferation and differentiation of MC3T3-E1 cells, respectively. the protein expressions of Dickkop (DKK-1), Osteoprotegerin (OPG), Osteopontin (OPN), Bone gla protein (BGP) and Receptor activator of NK-κB ligand (RANKL) were detected by suspension chip technology. Furthermore,the mRNA expressions of OPG and RANKL were evaluated by Real-time PCR.Results Isocoumarin glycoside increased the proliferation and differentiation of MC3T3-E1 cells. Further mechanism analysis showed that isocoumarin glycoside significantly promoted the protein expression of OPG, OPN and OPG/RANKL ratio. While the protein expression of DKK-1 and RANKL had no obvious change. mRNA results showed that isocoumarin glycoside obviously promoted the mRNA expression of OPG and OPG/RANKL ratio. Conclusion Isocoumarin glycoside increased MC3T3-E1 cells proliferation and differentiation through OPG/RANKL/RANK pathway, and and induce bone formation.

國家自然科學基金項目（81370096）；海南師范大學熱帶藥用植物化學教育部重點實驗室開放基金項目