目的 采用網(wǎng)絡(luò)藥理學(xué)的方法和思路研究葛根，探討葛根成分-靶點、成分-疾病、基因-通路和基因-疾病的關(guān)聯(lián)性，揭示其主要活性成分和藥理作用機(jī)制。方法 針對葛根中的活性成分，根據(jù)PubMed、中藥系統(tǒng)藥理學(xué)數(shù)據(jù)庫和分析平臺（TCMSP）數(shù)據(jù)庫中獲得葛根的化學(xué)成分和相關(guān)作用靶點，構(gòu)建"成分-靶點"網(wǎng)絡(luò)；運用UniProt和CTD數(shù)據(jù)庫提取作用靶點的基因名稱、信號通路、心血管疾病相關(guān)的基因和靶點數(shù)據(jù)進(jìn)行富集分析。結(jié)果 共獲得刺芒柄花素、葛根素等10個主要活性成分；這些成分可作用于112個靶點，其主要靶點有PTGS2、PTGS1、HSP90AB1等，這些靶點與腫瘤、高血壓、糖尿病等436種疾病相關(guān)，涵蓋腫瘤、心腦血管疾病等33大類疾病。葛根主要活性成分作用于心血管疾病的有48個靶點，這些靶點參與腫瘤、高血壓等多種生理及病理的生物學(xué)過程，如腫瘤壞死因子（TNF）等相關(guān)靶點，說明葛根是通過多成分、多靶點的協(xié)調(diào)作用發(fā)揮治療疾病的作用。結(jié)論 葛根具有抗腫瘤、降血壓、降血脂等生物活性，具有較好的應(yīng)用價值，為接下來的藥效試驗研究提供科學(xué)依據(jù)。

Objective To study the Pueraria lobata by system network pharmacology method, investigate the relations between main components-targets, targets-diseases and by analyzing the relationship of compounds-targets-gene-diseases, and reveal the multidimensional action pharmacology mechanism of Pueraria lobata. Methods The components of Pueraria lobata action targets and were obtained from TCMSP database and PubMed, and The components with OB ≥ 20% and DL ≥ 0.18 were the main active. The gene names of the targets, the pathways and corresponding diseases related with the targets were extracted from Uniprot database and CTD analysis platform online, respectively. At last, the components-targets, targets-diseases. Result The analysis of several networks pharmacological maps showed that The 10 active components were obtained, such as formononetin, puerarin, daidzein-4', 7-diglucoside, beta-sitosterol, 3'-methoxydaidzein, daidzein, daidzin, ononin. There were 112 targets related with the 10 active components, and the main targets were PTGS2, PTGS1, HSP90AB1 and so on. Those targets were related with 436 diseases including cancer, hypertension, diabetes mellitus, containing 33 kinds of diseases such as cancer, cardiovascular disease and so on. The main active components of Pueraria lobata have 48 targets acting on Cardiovascular disease. Those targets involved in various physiological and pathological processes such as cancer, Hypertension, Brain Ischemia, and inflammatory reaction in body, indicating that Pueraria lobata has multiple components and targets providing synergistic effects in the treatment of diseases. Conclusion Pueraria lobata has anti-cancer, lower blood pressure, and lower blood lipid avtivites, and could be used for treating cancer, cardiovascular disease, diabetes and so on, suggesting that Pueraria lobata has better application prospects.