目的 探討黃芪-川芎藥對(duì)治療腦卒中的藥理機(jī)制。方法 通過(guò)多個(gè)數(shù)據(jù)庫(kù)并結(jié)合文獻(xiàn)調(diào)研檢索黃芪、川芎的有效化學(xué)成分和相關(guān)靶點(diǎn)；采用Cytoscape軟件繪制成分靶點(diǎn)可視化網(wǎng)絡(luò)，并進(jìn)行拓?fù)浞治?；以CTD在線(xiàn)分析平臺(tái)挖掘腦卒中的相關(guān)靶點(diǎn)，借助STRING數(shù)據(jù)庫(kù)構(gòu)建化合物靶點(diǎn)蛋白互作網(wǎng)絡(luò)、腦卒中靶點(diǎn)蛋白互作網(wǎng)絡(luò)，應(yīng)用Cytoscape軟件進(jìn)行網(wǎng)絡(luò)合并獲取核心網(wǎng)絡(luò)，并對(duì)核心靶點(diǎn)基因進(jìn)行KEGG通路富集分析。結(jié)果 共篩選出黃芪、川芎中49個(gè)有效化合物，靶點(diǎn)蛋白272個(gè)，核心靶點(diǎn)蛋白39個(gè)，KEGG富集通路10條。作用通路涉及腫瘤壞死因子（TNF）信號(hào)通路、白介素17（IL-17）信號(hào)通路、松弛素信號(hào)通路、核因子（NF）-κB信號(hào)通路、低氧誘導(dǎo)因子-1（HIF-1）信號(hào)通路、C型凝集素受體信號(hào)通路、Toll樣信號(hào)通路、核苷酸結(jié)合寡聚化結(jié)構(gòu)域（NOD）樣信號(hào)通路、血管內(nèi)皮生長(zhǎng)因子（VEGF）信號(hào)通路、p53信號(hào)通路。結(jié)論 黃芪-川芎藥對(duì)中槲皮素、山柰酚等化合物可能通過(guò)腫瘤壞死因子（TNF）信號(hào)通路、白介素17（IL-17）信號(hào)通路等多條通路發(fā)揮治療腦卒中的作用。

Objective To investigate the mechanism of Astragalus Rhizoma-Chuanxiong in treatment of stroke. Methods The effective components and related target protein of Astragalus Rhizoma and Chuanxiong were screened by multiple databases and literature research. The effective compound-target protein visual network was established by using Cytoscape software, topology analysis was also performed. The targets related to stroke were found through CTD online analysis platform. The protein-protein interaction (PPI) network of targets related to compound and stroke were constructed and analyzed by STRING database. The two PPI networks were merged to acquire the core target, and KEGG pathway enrichment of target protein coding gene was analyzed by using Cytoscape software. Results A total of 49 kinds of effective compounds, 272 target proteins,39 core target proteins, and 10 enrichment pathways were selected. The target protein coding gene was rich in TNF signaling pathway, IL-17 signaling pathway, relaxin signaling pathway, NF-kappa B signaling pathway, HIF-1 signaling pathway, C-type lectin receptor signaling pathway, Tolllike receptor signaling pathway, NOD-like re ceptor signaling pathway, VEGF signaling pathway, p53 signaling pathway. Conclusion Quercetin and kaempferol of Astragalus Rhizoma and Chuanxiong play an effect on treatment of stroke through TNF signaling pathway, IL-17 signaling pathway and other signaling pathways.