目的 觀察杠板歸對(duì)小鼠H22肝癌的抑制作用，分析其可能的作用機(jī)制。方法 建立H22皮下移植小鼠模型評(píng)價(jià)杠板歸對(duì)腫瘤生長(zhǎng)的影響；二甲苯誘導(dǎo)的耳腫脹評(píng)價(jià)杠板歸對(duì)荷瘤小鼠炎癥的影響；網(wǎng)絡(luò)藥理學(xué)分析杠板歸抗腫瘤和抗炎可能的作用機(jī)制。結(jié)果 杠板歸對(duì)H22皮下腫瘤生長(zhǎng)有明顯抑制作用（P<0.05），顯著降低二甲苯誘導(dǎo)的荷瘤小鼠耳腫脹（P<0.05）。通過(guò)上海有機(jī)所化學(xué)專業(yè)數(shù)據(jù)庫(kù)以口服生物利用度（OB）≥30%和類藥性（DL）≥0.18篩選杠板歸4種有效成分，與生物芯片肝癌上下調(diào)基因蛋白分子對(duì)接得到106個(gè)預(yù)測(cè)靶點(diǎn)，預(yù)測(cè)靶點(diǎn)與TTD比對(duì)得到13個(gè)肝癌治療靶點(diǎn)。蛋白相互作用網(wǎng)絡(luò)（PPI）分析STAT3、IL-6、IL-1β、HBG2、TLR4、HBB、HBE1、HBD為8個(gè)核心靶點(diǎn)，其參與27個(gè)KEGG通路和27個(gè)生物過(guò)程。結(jié)論 杠板歸對(duì)小鼠H22肝癌有抑制作用，能減輕荷瘤小鼠炎癥，其作用與4個(gè)活性成分參與“泛素化介導(dǎo)蛋白水解”、“核因子（NF）-κB信號(hào)傳導(dǎo)”、“Janus激酶-信號(hào)轉(zhuǎn)導(dǎo)與轉(zhuǎn)錄激活子（JAK-STAT）信號(hào)傳導(dǎo)”等通路有關(guān)。

Objective To observe the inhibitory effect of Polygonum perfoliatum L. on H22 liver carcinoma in mice and explore its possible action mechanisms. Methods Effect of Polygonum perfoliatum L. on tumor growth was evaluated using H22 subcutaneously transplanted tumor model in mice, effect of Polygonum perfoliatum L on inflammation was examined by ear edema in tumor bearing mice. Network pharmacology was used to analyze the possible action mechanisms by which Polygonum perfoliatum L. exerts anti-tumor and anti-inflammation efficacy. Results Polygonum perfoliatum L. had an anti-tumor effect on H22 liver carcinoma in mice and obviously decreased xylene-induced ear edema in tumor-bearing mice(P<0.05). 4 active components with OB ≥ 30%and DL ≥ 0.18 in Shanghai Institute of Organic Chemistry database, and up-and-down genes of liver cancer in the biochip were selected for molecular docking. 106 predicted targets were compared to liver cancer therapeutic targets in TTD, there were 13 liver cancer therapeutic targets related with four active components. PPI analysis indicates that STAT3, IL6, IL1B, HBG2, TLR4, HBB, HBE1, HBD are 8 core targets which involve in 27 KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways and 27 GO (gene ontology) biological process. Conclusion Polygonum perfoliatum L. has an inhibitory effect on H22 liver cancer and could decrease inflammation in tumor bearing mice, its action mechanisms are associated 4 active components which involve in "ubiquitination-mediated proteolysis", "NF-κB signaling", and "JAK-STAT signaling", et al pathway.

河南省自然科學(xué)基金項(xiàng)目（182300410310）