目的 建立臨床前毒理學(xué)研究中Beagle犬中樞神經(jīng)系統(tǒng)一致性制片方法。方法 以Beagle犬腦組織肉眼可見的解剖部位，如額極、視交叉、動(dòng)眼神經(jīng)、腦橋、前庭耳蝸神經(jīng)為標(biāo)志，對(duì)Beagle犬腦組織（腹側(cè)面向上）進(jìn)行冠狀水平單側(cè)取材。并且對(duì)Beagle犬脊髓的顱頸段（C1~C2）、胸中段（T6~T8）和腰膨大處（L4~L5）進(jìn)行橫切面及傾斜橫向面取材，并經(jīng)常規(guī)脫水、包埋、切片、HE染色處理。結(jié)果 通過(guò)這種方法制作的Beagle犬中樞神經(jīng)系統(tǒng)組織學(xué)切片一致性高、質(zhì)量好，包括了Beagle犬中樞神經(jīng)系統(tǒng)主要結(jié)構(gòu)，例如尾狀核、基底核、大腦皮質(zhì)（額葉、頂葉、枕葉、顳葉）、脈絡(luò)叢、海馬、下丘腦、延髓、中腦、腦橋、丘腦、小腦和脊髓。結(jié)論 該制片方法簡(jiǎn)單易行且一致性高，可用于臨床前毒理學(xué)研究中Beagle犬中樞神經(jīng)系統(tǒng)組織病理學(xué)檢查的一致性制片。

Objective To establish a consistent slide preparation method of Beagle dog central nervous system (CNS) for preclinical toxicology studies. Methods The anatomical parts visible outside the brain tissue, such as the frontal pole, optic chiasm, oculomotor nerve, pons, and vestibular cochlear nerve were used as landmarks. Unilateral sampling was performed at the coronal horizontal level of the brain tissue of Beagle dog (ventral side up). Moreover, cross sections and oblique transverse sections were obtained from the cranio cervical segments of spinal cord (C1-C2), middle thoracic segment (T6-T8) and lumbar enlargement (L4-L5) of Beagle dog. The samples were routinely dehydrated, embedded, sectioned and HE stained. Results The histological sections of the CNS of Beagle dog were produced with high quality and good consistency and contained major structures of CNS, such as the caudate nucleus, basal ganglia, cerebral cortex (frontal lobe, parietal lobe, occipital lobe and temporal lobe), choroid plexus, hippocampus, hypothalamus, medulla oblongata, midbrain, pons, thalamus, cerebellum and spinal cord. Conclusion The method is easy and consistent and can be used for preparation of the consistent slides for histopathological examination of the CNS of Beagle dog in preclinical toxicology studies.

國(guó)家"重大新藥創(chuàng)制"科技重大專項(xiàng)課題（2018ZX09201-017）