[關鍵詞]
[摘要]
目的 研究有氧運動對小鼠主動脈自噬相關因子表達及自噬活化的影響。方法 將8周齡C57BL/6雄性小鼠40只隨機分為安靜對照組(n=20)與有氧運動組(n=20),其中10只用于Real-Time PCR實驗,10只用于Western-blot實驗,有氧運動組小鼠進行6周的游泳運動(60 min/d,5 d/周),對照組小鼠自由活動。6周后取小鼠胸腹主動脈,采用Real-Time PCR方法檢測自噬相關因子ULK1、Beclin1、LC3B mRNA水平;Western-blot方法檢測主動脈LC3B蛋白中LC3B-II/LC3B-Ⅰ比值及p62蛋白水平。結果 與對照組相比,有氧運動組小鼠主動脈ULK1、Beclin1、LC3B mRNA水平明顯升高(P<0.05);有氧運動組小鼠主動脈LC3B-II/LC3B-Ⅰ比值增大(P<0.05),p62蛋白降解增加(P<0.05)。結論 有氧運動能夠使主動脈ULK1、Beclin1、LC3B自噬相關因子表達增強,進而增強主動脈自噬活性。
[Key word]
[Abstract]
Objective To investigate the effect of aerobic exercise on the levels of autophagy-related markers and Autophagy activation in mouse aorta. Methods Twenty C57BL/6 male mice aged 8 weeks were randomly divided into sedentary control group and aerobic exercise group. The aerobic exercise group were subjected to swimming for 6 weeks, 60 min/d, 5 days a week. The Thoracic and abdominal aorta of mice were isolated and detected after 6 weeks. Real-Time PCR was adopted to measure the mRNA levels of autophagy-related markers, including ULK1, Beclin1 and LC3B. Western-blot was applied to measure the expression level of autophagy-related protein including the ratio of LC3B-II/LC3B-Ⅰ and p62. Results Compared with the control group, in the aerobic exercise group, the mRNA levels of ULK1, Beclin1 and LC3B and the ratio of LC3B-II/LC3B-Ⅰ were significantly increased (P<0.05), the protein degradation of p62 was significantly increased (P<0.05). Conclusion Aerobic exercise can increase the expression of ULK1, Beclin1 and LC3B in the aorta, and then enhance the aortic autophagy activity.
[中圖分類號]
[基金項目]
山東省高等學校科技計劃項目(J18KB117);濱州醫(yī)學院附屬醫(yī)院青年科研基金(2013QNKYJJ02)