目的 觀察姜黃素對(duì)大鼠腸缺血再灌注后腸黏膜細(xì)胞凋亡及GRP78表達(dá)的影響。方法 30只雄性SD大鼠隨機(jī)分為假手術(shù)組、模型組、姜黃素組。采用無(wú)創(chuàng)性動(dòng)脈夾夾閉腸系膜上動(dòng)脈1 h后實(shí)施再灌注來建立腸缺血再灌注模型，姜黃素組在手術(shù)前5 d開始每天按200 mg/kg ig給藥1次，假手術(shù)組僅分離腸系膜上動(dòng)脈，不夾閉。采用HE染色觀察腸黏膜損傷情況并進(jìn)行病理評(píng)分；TUNEl法觀察腸黏膜細(xì)胞凋亡情況并計(jì)算凋亡指數(shù)；Western blotting檢測(cè)再灌注損傷3 h時(shí)腸黏膜GRP78及Caspase-12蛋白表達(dá)。結(jié)果 模型組腸黏膜損傷嚴(yán)重，絨毛上皮成塊脫落，固有層破壞、潰瘍，腺體破壞嚴(yán)重；姜黃素組腸黏膜絨毛上皮僅部分脫落，腺體排列紊亂，與模型組比較，病理評(píng)分顯著降低（P<0.05）；模型組腸黏膜隱窩可見大量凋亡陽(yáng)性細(xì)胞；與模型組比較，姜黃素組腸黏膜隱窩上皮細(xì)胞凋亡情況明顯減輕，凋亡指數(shù)顯著下降（P<0.05）；與模型組比較，姜黃素組GRP78蛋白表達(dá)量顯著增加（P<0.05），Caspase-12蛋白表達(dá)量顯著下降（P<0.05）。結(jié)論 姜黃素有可能通過上調(diào)GRP78表達(dá)，減少內(nèi)質(zhì)網(wǎng)應(yīng)激所致腸黏膜細(xì)胞凋亡，來減輕腸黏膜的再灌注損傷，達(dá)到保護(hù)腸黏膜作用。

Objective To observe the possible effect of curcumin on GRP78 expression and epithelial cells apoptosis in rats after intestinal ischemia reperfusion. Method Thirty male SD rats were randomly divided into three groups: sham group, model group and curcumin group, 10 rats in each group. An experimental model of intestinal ischemia reperfusion in rats was established by the occlusion of superior mesenteric artery for one hours by small atraumatic clip, followed by reperfusing, and sham group were prepared in the same way without the occlusion. Curcumin group were feeded curcumin at a dose of 200 mg/kg per day 5 days before operating. Intestinal tissue injury severity of rat was tested by HE assay. The apoptosis of epithelial cells was measured by TUNEL method. The expression of GRP78 in rats tissue after 3 h reperfusion was detected by Western blotting. Results In the model group, intestinal mucosa was damaged seriously, villous epithelium was exfoliated, lamina propria was damaged, ulcerated and glands were damaged seriously. Compared with the model group, the pathological score of curcumin group decreased significantly (P < 0.05). Compared with the model group, the apoptosis of intestinal mucosal crypt epithelial cells in curcumin group decreased significantly (P < 0.05). Compared with the model group, the expression of GRP78 and caspase-12 in curcumin group increased significantly (P < 0.05). Conclusion Curcumin could reduce intestinal ischemia reperfusion injury in rats by up-regulation GRP78 expression.

R322.45;R656.7