目的 采用網(wǎng)絡藥理學方法探討痰咳凈方治療呼吸系統(tǒng)的潛在作用機制，并對其防治新型冠狀病毒肺炎（COVID-19）的可能性進行評估。方法 通過TCMSP、Swiss Target Prediction、OMIM等在線數(shù)據(jù)庫收集痰咳凈方有效化學成分、成分作用靶點及相關疾病信息。采用Cytoscape 3.7.1構建化合物-靶點網(wǎng)絡和靶點-疾病網(wǎng)絡。將上述靶點用String 10.0數(shù)據(jù)庫進行蛋白質(zhì)-蛋白質(zhì)相互作用（PPI）網(wǎng)絡的構建并篩選出核心靶點，最后利用DAVID 6.8數(shù)據(jù)庫進行基因本體（GO）功能富集分析和KEGG通路富集分析，預測其作用機制。結果 共篩選出49個主要活性成分，與呼吸系統(tǒng)相關靶點85個，針對疾病19種。PPI網(wǎng)絡發(fā)現(xiàn)關聯(lián)度較高的靶點有AKT1、IL-6、MAPK1、PTGS2、VEGFA、TNF、EGFR、STAT3、CCND1、MMP9、JUN、IL-10、ESR1等?；虮倔w（GO）條目1 793個，其中生物過程相關的條目1 555個，分子功能相關的條目134個，細胞組成相關的條目104個。靶點對應的信號通路為137條，涉及炎癥性通路、病毒相關通路、免疫調(diào)節(jié)性通路、信號轉(zhuǎn)導性通路、腫瘤相關通路及內(nèi)分泌代謝通路等。結論 研究初步證明痰咳凈方通過作用于ADCY2、AKT1、CASP8、CCND1、TNF、CASP8、IFNG、IL-4、IL-6等靶點調(diào)節(jié)人巨細胞病毒感染通路、IL-17信號通路、FoxO信號通路、HIF-1信號通路、人T細胞白血病病毒感染、TNF信號通路等多條信號通路，來發(fā)揮對COVID-19的防治作用。

Objective To study the potential mechanism of Tankejing in the treatment of respiratory system based on network pharmacology, and to evaluate the possibility of Tankejing in the prevention and treatment of COVID-19 pneumonia. Methods The effective chemical components, targets and related diseases of Tankejing were collected through online databases such as TCMSP, Swiss Target Prediction and OMIM. The relationship of Compound-Target network and Target-Disease network was established base on the Cytoscape 3.7.1. The above targets were constructed using String10.0 database for the PPI network and screen out the key targets. Finally, using the DAVID 6.8 database to perform GO enrichment analysis and KEGG pathway enrichment analysis and to predict the action mechanism of Tankejing. Results There were 49 active components of Tankejing with 85 targets sites related to respiratory system, 94 of directly acting on disease. The PPI network found high correlation targets including AKT1, IL6, MAPK1, PTGS2, VEGFA,TNF, EGFR, STAT3, CCND1, MMP9, JUN, IL10, ESR1, etc. There were 1,793 entries in the gene ontology (GO), including 1, 555 entries related to biological processes, 134 entries related to molecular functions, and 104 entries related to cell composition. There were 137 signaling pathways corresponding to the target, involving inflammatory pathways, virus-related pathways, immunoregulatory pathways, signal transduction pathways, tumor-related pathways, endocrine and metabolic pathways, etc. Conclusion This study preliminarily proves that Tankejing can play a role in the prevention and treatment of COVID-19 by acting on adcy2, AKT1, CASP8, CCND1, TNF, CASP8, IFNG, IL4, IL6 and other targets to regulate human cytomegalovirus infection pathway, IL-17 signaling pathway, FOXO signaling pathway, HIF-1 signaling pathway, human T cell leukemia virus 1 infection, TNF signaling pathway and other signaling pathways.

R285.5

國家科技重大專項：中醫(yī)藥優(yōu)勢領域的創(chuàng)新中藥關鍵技術開發(fā)研究（2017ZX09301005）；山東省重點研發(fā)計劃：新型冠狀病毒潛在藥物臨床評價研究（2020SFXGFY07）