目的 研究染料木素對野百合堿引起的肺動脈高血壓（PH）模型大鼠的降壓作用及機(jī)制。方法 大鼠隨機(jī)分組后，模型組及染料木素組大鼠sc 50 mg/kg野百合堿制備PH模型，對照組sc生理鹽水。野百合堿注射后第3天，染料木素低、中、高劑量組ig染料木素10、50、100 mg/kg，對照組和模型組大鼠ig給予等體積生理鹽水，1次/d，連續(xù)2周。末次給藥后，采用尾套法測量清醒大鼠尾部動脈血壓；小動物生理儀檢測大鼠平均心室壓和收縮壓；熒光實(shí)時(shí)定量PCR（RT-qPCR）檢測血清白細(xì)胞介素（IL）-6、IL-8、IL-1β、腫瘤壞死因子-α（TNF-α）、轉(zhuǎn)化生長因子-β（TGF-β）水平；試劑盒測定大鼠血清超氧化物歧化酶（SOD）值及丙二醛（MDA）、總抗氧化能力（T-AOC）和活性氧（ROS）水平。結(jié)果 與模型組比較，染料木素50、100 mg/kg組動脈血壓、右心室收縮壓（RVSP）和右心室舒張末期壓（RVeDP）均顯著下降（P<0.05、0.01）；染料木素10、50、100 mg/kg劑量均可顯著降低大鼠血清中IL-6、IL-8、IL-1β、TNF-α、TGF-β的表達(dá)（P<0.05、0.01）；染料木素10、50、100 mg/kg劑量組ROS和MDA水平顯著降低，而T-AOC和SOD水平顯著增加（P<0.05、0.01）。結(jié)論 染料木素對野百合堿誘導(dǎo)的PH大鼠具有降低血壓的作用，其機(jī)制可能與其阻斷氧化應(yīng)激有關(guān)。

Objective To study the antihypertensive effect of genistein on monocrotaline-induced pulmonary hypertension (PH) in rats and its mechanism. Methods Rats in the model group and genistein treatment group were randomly divided into two groups. On the first day of the experiment, the rats in the model group and genistein treatment group were subcutaneously injected with monocrotaline at a concentration of 50 mg/kg. The rats in the control group were subcutaneously injected with normal saline. Fifth week after the administration, the rats in the genistein treatment group were orally given genistein of 10, 50, 100 mg/kg. Then the rats in the control group and the model group were randomly divided into two groups and given distilled water or genistein orally to record blood pressure and renal sympathetic nerve activity. The effects of genistein on blood pressure, right ventricular systolic pressure, right ventricular mean pressure and right ventricular hypertrophy index were detected; the effects of genistein on IL-6, IL-8, IL-1β, TNF-α and TGF-β in serum of rats were detected by PCR; the levels of ROS, MDA, T-AOC and SOD in serum were determined. Results Compared with the model group, the levels of arterial blood pressure, right ventricular systolic pressure (RVSP) and right ventricular end diastolic pressure (RVEDP) of Genistein 50, 100 mg/kg group decreased significantly (P<0.05, 0.01), and the levels of IL-6, IL-8, IL-1β, TNF-α, TGF-β of Genistein 10, 50 and 100 mg/kg group in serum decreased significantly (P<0.05, 0.01), the levels of ROS and MDA decreased of Genistein 10, 50 and 100 mg/kg group significantly, while the levels of T-AOC and SOD increased significantly (P<0.05, 0.01). Conclusion Genistein can reduce blood pressure in hypertensive rats induced by monocrotaline, and the mechanism may be related to blocking oxidative stress.

R285.5

河南省衛(wèi)計(jì)委科研基金項(xiàng)目（2018020783）