目的 探究七氟醚對(duì)急性心肌缺血再灌注（AIRI）模型的老齡大鼠血清5-羥色胺（5-HT）、白介素（IL）-1α、IL-1β和海馬組織超氧化物歧化酶（SOD）、丙二醛（MDA）的影響。方法 選取SPF級(jí)健康雄性老齡大鼠40只，隨機(jī)分為5組，建立AIRI模型，模型組給予氧氣吸入，七氟醚組分別吸入1%、2%、3%及4%的七氟醚，采用免疫組織化學(xué)法檢測(cè)大鼠心臟組織5-HT、IL-1α、IL-1β的蛋白表達(dá)量；ELISA法檢測(cè)血清5-HT、IL-1α、IL-1β濃度；采用黃嘌呤氧化酶法測(cè)定海馬組織SOD的活性，硫代巴比妥酸顯色法測(cè)定海馬組織MDA活性；實(shí)時(shí)熒光定量PCR（RT-qPCR）法檢測(cè)血清5-HT、IL-1α、IL-1β和海馬組織SOD、MDA中mRNA的表達(dá)量。結(jié)果 與模型組比較，七氟醚2%、3%、4%濃度組心臟組織、血清中5-HT、IL-1α、IL-1β蛋白水平、血清中5-HT、IL-1α、IL-1β mRNA水平顯著降低（P<0.05）；七氟醚2%、3%、4%濃度組海馬組織MDA蛋白及mRNA水平顯著降低，SOD蛋白及mRNA水平顯著升高（P<0.05）。結(jié)論 2%~4%的七氟醚能夠顯著降低AIRI模型老齡大鼠心臟、血清5-HT、IL-1α、IL-1β表達(dá)水平，降低海馬組織MDA水平，提高SOD活性。

Objective To investigate the effects of sevoflurane concentration on serum 5-hydroxytryptamine (5-HT), interleukin (IL) and malondialdehyde (MDA) in hippocampal tissue of aged rats with acute myocardial ischemia-reperfusion (AIRI) model. Methods Totally 40 healthy male aged rats of SPF grade were randomly divided into five groups with eight rats in each group. The rats model of AIRI was established. Rats in control group was inhaled with oxygen, the sevoflurane group inhaled 1%, 2%, 3% and 4% sevoflurane respectively. The protein expression of 5-HT, IL-1α and IL-1β in serum of rats inhaled with different concentrations of sevoflurane was detected by immunohistochemistry, and the concentration of sevoflurane was detected by ELISA. At the same time, the activity of SOD was determined by xanthine oxidase method and the activity of MDA was determined by thiobarbituric acid colorimetric method. The expression of mRNA of 5-HT, IL-1α and IL-1β and hippocampal SOD, MDA was detected by RTqPCR. Results Compared with model group, the levels of 5-HT, IL-1 α, IL-1 β protein and mRNA in the heart and serum of the group with 2%, 3% and 4% sevoflurane decreased significantly (P<0.05); the levels of MDA protein and mRNA in the hippocampus of the group with 2%, 3% and 4% sevoflurane decreased significantly, and the levels of SOD protein and mRNA increased significantly (P<0.05). Conclusion 2%-4% sevoflurane can significantly reduce the expression level of 5-HT, IL-1 α, IL-1β in the heart and serum of aging rats with AIRi model, reduce the MDA level in hippocampus and improve the activity of SOD.

R965.1

河南省科技發(fā)展計(jì)劃（152300410163）