目的 從藥效學(xué)角度探討甘草-大戟配伍禁忌的機(jī)制。方法 30只SD大鼠按體質(zhì)量隨機(jī)分成6組，每組5只，即大戟單煎液組、甘草單煎液組、甘草-大戟（1：1、2：1、3：1）合煎液組和對(duì)照組，分別ig等體積大戟單煎液（0.24 g/kg）、甘草單煎液（0.72 g/kg）、甘草-大戟1：1合煎液（甘草0.24 g/kg、大戟0.24 g/kg）、甘草-大戟2：1合煎液（甘草0.48 g/kg、大戟0.24 g/kg）、甘草-大戟3：1合煎液（甘草0.72 g/kg、大戟0.24 g/kg）和等體積生理鹽水，大戟、甘草單煎液劑量設(shè)置參考《中國(guó)藥典》規(guī)定的人口服劑量，根據(jù)人和大鼠體表面積比例進(jìn)行換算。觀察大鼠的排尿量、尿液中總蛋白含量、排便量、小腸推進(jìn)率，考察甘草對(duì)大戟峻下逐水作用的影響。結(jié)果 與大戟單煎液組比較，甘草-大戟合煎液組大鼠的排尿量和排便量減少，小腸推進(jìn)率降低，尿液中總蛋白濃度升高，其中，甘草-大戟（1：1、2：1、3：1）合煎液組大鼠的排便量均顯著減少（P<0.05、0.01），甘草-大戟（1：1、3：1）合煎液組大鼠的排尿量顯著減少（P<0.01），甘草-大戟（2：1、3：1）合煎液組大鼠的小腸推進(jìn)率顯著降低（P<0.01），甘草-大戟（2：1、3：1）合煎液組大鼠尿液中總蛋白濃度顯著升高（P<0.01）。結(jié)論 甘草與大戟配伍可明顯拮抗大戟的利尿和瀉下作用，影響大戟的峻下逐水功效。

Objective To investigate the pharmacodynamic incompatibility of Glycyrrhizae Radix with Euphorbiae Pekinensis Radix. Methods Tatolly thirty SD rats were randomly divided into six groups according to body weight, with five rats in each group, namely, Euphorbiae Pekinensis single decoction group, Radix Glycyrrhizae single decoction group, Radix Glycyrrhizae-Euphorbiae Pekinensis (1:1, 2:1, 3:1) combined decoction group, and control group. The same volume of Euphorbiae Pekinensis single decoction (0.24 g/kg), Radix Glycyrrhizae single decoction (0.72 g/kg), Radix Glycyrrhizae-Euphorbiae Pekinensis (1:1) combined decoction (0.24 g/kg + 0.24 g/kg), Radix Glycyrrhizae-Euphorbiae Pekinensis (2:1) combined decoction (0.48 g/kg + 0.24 g/kg),Radix Glycyrrhizae-Euphorbiae Pekinensis (3:1) combined decoction (0.72 g/kg + 0.24 g/kg), and normal saline were ig to relative group. In order to investigate the influence of Radix Glycyrrhizae on the drastic diuretic effect of Euphorbiae Pekinensis, observed the urine output, total protein content in the urine, defecation amount and small intestinal propulsive rate of rats. Results Compared with the Euphorbiae Pekinensis single decoction group, all of the Radix Glycyrrhizae-Euphorbiae Pekinensis (1:1, 2:1, 3:1) groups showed less urine output and defecation amount, lower intestinal propulsive rate and higher urinary protein concentration. Compared with the Euphorbiae Pekinensis single decoction group, All of the Radix Glycyrrhizae-Euphorbiae Pekinensis (1:1, 2:1, 3:1) groups had significantly less defecation amount (P<0.05 and 0.01), a part of the Radix Glycyrrhizae -Euphorbiae Pekinensis (1:1, 3:1) groups had significantly less urine output (P<0.01), a part of the Radix Glycyrrhizae-Euphorbiae Pekinensis (2:1, 3:1) groups showed significantly lower intestinal propulsive rate (P<0.01), and a part of the Radix Glycyrrhizae-Euphorbiae Pekinensis (2:1, 3:1) groups exhibited significantly higher urinary protein concentration (P<0.01). Conclusion The concomitant using Radix Glycyrrhizae can obviously suppress the diuretic and purgative effects of Euphorbiae Pekinensis, and influence its drastic diuretic efficacy.

R285.5

國(guó)家科技重大專項(xiàng)（2019ZX09201004-002）