目的 基于中醫(yī)血熱證的病因，復合銀屑病癥狀，建立銀屑病病證結(jié)合的豚鼠復合模型。方法 采用多因素復合造模法，ig給予豚鼠復方熱藥造成血熱證模型，豚鼠耳部皮膚涂抹5%鹽酸普萘洛爾乳劑造成銀屑病模型，從體征、血流變、肛溫及耳部皮膚病理變化對模型進行評價，并采用消銀片（2.1 g/kg）對其進行反證。結(jié)果 與對照組比較，模型組豚鼠出現(xiàn)典型的血熱證銀屑病癥狀，肛溫明顯升高（P<0.05、0.01），全血黏度低切（LS）、中切（MS）、高切（HS）值均顯著升高（P<0.01），耳組織質(zhì)量和厚度均顯著增加（P<0.05、0.01）；Baker評分及炎細胞浸潤數(shù)顯著升高（P<0.01）；肉眼觀察，模型組豚鼠耳部潮紅，皮膚表面形成結(jié)痂，出現(xiàn)白色銀屑，觸之耳組織表面粗糙、質(zhì)地變硬、厚度增加；鏡檢結(jié)果顯示，模型組豚鼠皮膚角化過度形成較厚的結(jié)痂，顆粒層不完整，棘層顯著增厚，表皮突下延，乳突伸長呈杵狀，新生毛細血管不同程度增多且血管充血，伴有不同程度水腫及炎細胞浸潤。與模型組比較，消銀片組右耳厚度及質(zhì)量，耳組織平均厚度及平均質(zhì)量均顯著下降（P<0.05）；Baker評分及炎細胞浸潤數(shù)均顯著降低（P<0.05）。結(jié)論 豚鼠血熱證銀屑病模型既有銀屑病的病理特征，又具有中醫(yī)血熱證的證候表現(xiàn)，是適用于中藥的療效評價和作用機制研究的實驗動物模型。

Objective Based on the etiology of the blood-heat syndrome of traditional Chinese medicine in psoriasis and the combination of the symptoms of psoriasis, the guinea pig composite model of psoriasis syndrome was established. Methods The method of muti-facto compound molding was adopted, Model of blood-heat Syndrome induced by ig of compound hot medicine in Guinea Pigs, Ears' backs of guinea pigs were treated topically with 5% propranolol emulsion to create a psoriasis model,The model was evaluated by physical signs, blood rheology, anal temperature and pathological changes of ear skin. Xiaoyin tablets (2.1 g/kg) were used to prove it. Results Compared with control group, guinea pigs in model group had typical symptoms of psoriasis of blood heat syndrome, anal temperature was significantly increased (P<0.05, 0.01), whole blood viscosity low cut (LS), middle cut (MS), high cut (HS) values were significantly increased (P<0.01), ear tissue mass and thickness were significantly increased (P<0.05, 0.01); Baker score and inflammatory cell infiltration number were significantly increased (P<0.01). Naked eye observation showed that the ears of guinea pigs in the model group were flushed, scabs were formed on the surface of the skin, and white silver filings appeared on the surface of the skin. The surface of ear tissue was rough, the texture became hard and the thickness increased. The results of microscopic examination showed that in the model group, the skin hyperkeratosis formed thick scab, the granular layer was incomplete, the spinous layer was significantly thickened, the epidermal process was extended downward, the mastoid process was elongated like a pestle, the number of new capillaries increased in varying degrees and the blood vessels were congested, accompanied by edema and inflammatory cell infiltration in different degrees. Compared with the model group, the thickness and weight of right ear, average thickness and average mass of ear tissue in Xiaoyin tablet group were significantly decreased (P<0.05), Baker score and inflammatory cell infiltration number were significantly decreased (P<0.05). Conclusion The Guinea pig model of psoriasis with blood-heat syndrome has not only the pathological features of psoriasis, but also the syndrome manifestations of blood-heat syndrome, An experimental animal model for the efficacy evaluation and mechanism of traditional Chinese medicine in the treatment of psoriasis.

R965.1

山西省科技廳實驗動物專項基金項目（2015K07）