目的 研究甘草酸銨對(duì)特應(yīng)性皮炎（AD）小鼠IL-33/ST2通路及肥大細(xì)胞的活化情況的影響。方法 72只ICR小鼠（雌雄各半）隨機(jī)均分為對(duì)照組、模型組及甘草酸銨低、中、高劑量（25、50、100 mg/kg）和潑尼松龍（陽(yáng)性藥，60 mg/kg）組。除對(duì)照組外，以丙酮-DNCB為致敏源構(gòu)建AD小鼠模型，建模后各組ip相應(yīng)藥物，對(duì)照組和模型組ip生理鹽水。記錄小鼠夜間搔抓次數(shù)；甲苯胺藍(lán)患處皮膚組織染色法觀察肥大細(xì)胞活化情況；ELISA法檢測(cè)白細(xì)胞介素（IL）-33和ST2血清學(xué)水平；實(shí)時(shí)熒光定量PCR（qRT-PCR）和Weston blotting法檢測(cè)皮膚組織中的IL-33和ST2 mRNA和蛋白水平。結(jié)果 與對(duì)照組比較，模型組小鼠的搔抓次數(shù)顯著增多（P<0.05），肥大細(xì)胞密度顯著升高（P<0.05），IL-33和ST2的血清學(xué)水平、皮膚組織的轉(zhuǎn)錄水平和蛋白表達(dá)水平均顯著升高（P<0.05）；與模型組比較，潑尼松龍組和甘草酸銨低、中、高劑量組的搔抓次數(shù)和肥大細(xì)胞密度分別顯著減少和降低（P<0.05），IL-33、ST2的血清學(xué)水平、皮膚組織mRNA和蛋白表達(dá)均顯著降低（P<0.05）。結(jié)論 甘草酸銨能夠明顯抑制AD模型小鼠IL-33和ST2的血清學(xué)水平、皮膚組織中的轉(zhuǎn)錄水平和蛋白表達(dá)，降低了肥大細(xì)胞的活化程度，從而減輕AD的瘙癢癥狀。

Objective To investigate the effect of ammonium glycyrrhizinate on the IL-33/ST2 pathway and the activation of mast cells in atopic dermatitis (AD) mice. Methods Tatolly 72 ICR mice (male and female) were randomly divided into control group, model group, ammonium glycyrrhizinate low, medium, high dose (25, 50, and 100 mg/kg) group, and bonisonone (positive drug, 60 mg/kg) group. In addition to control group, acetone-DNCB was used as the sensitization source to construct the AD mouse model. After the modeling, normal saline was ip into the abdomen of control and the model group. The corresponding drugs were ip to mice of each group. The number of scratches were count at night. The activation of mast cells in the skin tissue was observed by skin tissuetoluidine stained. The serum levels of IL-33 and ST2 were detected by ELISA; the mRNA and protein levels of IL-33 and ST2 in skin tissue were detected by real-time fluorescent quantitative PCR (qRT-PCR) and Weston blotting. Results Compared with control group, the scratching times of mice in model group increased significantly (P<0.05), mast cell density increased significantly (P<0.05), serum, transcription and protein expression levels of IL-33 and ST2 were significantly increased in the model group (P<0.05). Compared with model group, scratching times and mast cell density were significantly reduced in the bonisonone group and the ammonium glycyrrhizinate low, medium and high groups, respectively (P<0.05). The serological level, mRNA and protein expression of IL-33 and ST2 were significantly reduced (P<0.05). Conclusion Ammonium glycyrrhizinate can significantly inhibit the transcription level and protein expression of IL-33 and ST2 in serum and tissues of AD model mice, reduce the activation degree of mast cells, and thus relieve the itch symptoms of AD.

R965

南充市市校戰(zhàn)略合作科技項(xiàng)目（18SXHZ0133）