目的 探討三子養(yǎng)親湯（SZ）對大鼠實(shí)驗(yàn)性支氣管哮喘的保護(hù)作用及潛在機(jī)制。方法 以卵清蛋白（OVA）致敏和激發(fā)誘發(fā)支氣管哮喘，將大鼠隨機(jī)分為5組：對照組、模型組、地塞米松（陽性藥，1 mg/kg）組、SZ治療（200 mg/kg）組和SZ單給藥（不造模，200 mg/kg）組。試劑盒法檢測血清免疫球蛋白E（IgE）水平。ELISA試劑盒法檢測支氣管肺泡灌洗液（BALF）中的炎性生物標(biāo)志物白細(xì)胞介素（IL）-4、IL-5、IL-13、腫瘤壞死因子-α（TNF-α）、γ-干擾素（IFN-γ）；Dunger's稀釋液染色法進(jìn)行BALF中嗜酸性粒細(xì)胞計數(shù)；試劑盒法檢測BALF中氧化氮應(yīng)激生物標(biāo)記物丙二醛（MDA）和谷胱甘肽（GSH）水平、超氧化物歧化酶（SOD）的活性；實(shí)時熒光定量PCR法檢測BALF中誘導(dǎo)型一氧化氮合酶（iNOs）RNA水平。以Western blotting法檢測肺勻漿中肺表面活性蛋白-D（SP-D）表達(dá)水平；免疫組化測定肺中尿皮質(zhì)素（UCN）的表達(dá)。HE染色法觀察大鼠肺組織病理學(xué)變化。結(jié)果 與模型組比較，SZ治療可顯著降低血清IgE水平（P<0.05）；顯著降低BALF中MDA含量，同時顯著提高SOD活性和GSH含量（P<0.05）；顯著降低BALF中IL-4、IL-5、IL-13、IFN-γ和TNF-α水平（P<0.05）；顯著降低肺組織中SP-D和UCN的蛋白表達(dá)（P<0.05）；HE染色結(jié)果表明，SZ顯著改善模型大鼠肺組織炎癥病變。結(jié)論 SZ可通過下調(diào)SP-D和UCN表達(dá)改善OVA誘導(dǎo)的支氣管哮喘。

Objective Investigate the protective effect and mechanism of Sanziyangqin Decoction (SZ) on experimental bronchial asthma in rats. Methods Bronchial asthma was sensitized and stimulated with ovalbumin (OVA). Rats were randomly divided into five groups, including normal control group, model group, DEXA (positive drug, 1 mg/kg) group, SZ treatment (200 mg/kg) and SZ control (200 mg/kg) group. The level of serum immunoglobulin E (IgE) was detected by kit method. Interleukin (IL)-4, IL-5, IL-13, tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in bronchoalveolar lavage fluid (BALF) were detected by ELISA kit; Dunger's dilution staining method was used to count eosinophils in BALF; the levels of malondialdehyde (MDA), glutathione (GSH) and the activity of superoxide dismutase (SOD) in BALF were detected by kit method; the level of inducible nitric oxide synthase (iNOS) RNA in BALF was detected by real-time quantitative PCR. The expression of pulmonary surfactant protein-D (SP-D) in lung homogenate was detected by Western blotting, and the expression of urinary corticosteroids (UCN) was detected by immunohistochemistry. He staining was used to observe the pathological changes of lung tissue. Results Compared with the model group, SZ treatment significantly reduced the serum IgE level (P<0.05), significantly reduced the MDA content in BALF, significantly increased the SOD activity and GSH content (P<0.05), significantly reduced the levels of IL-4, IL-5, IL-13, IFN-γ and TNF-α in BALF (P<0.05), significantly reduced the protein expression of SP-D and UCN in lung tissue (P<0.05); HE staining showed that SZ significantly improved the model inflammatory changes of lung tissue in model rats. Conclusion SZ can improve the bronchial asthma induced by ovalbumin by regulating the expression of SP-D and UCN.

R965

西安市科技計劃項目（201805103YX11SF37）