干細(xì)胞藥物臨床前研究中，常需要以干細(xì)胞特定基因片段為靶點追蹤其體內(nèi)的分布過程，需要提取動物樣品基因組DNA，涉及到心臟、肝臟、肺臟、脾臟、腎臟、腦、骨髓、血、脂肪、肌肉、性腺、胃和腸等多種組織類型。為實現(xiàn)臨床前樣品的高通量檢測，總結(jié)動物組織基因組DNA提取方法，并就如何高效高質(zhì)提取樣品基因組DNA進(jìn)行討論，提出間充質(zhì)干細(xì)胞臨床前研究中樣品處理的一種可行的方案：通過生物樣品制備儀獲取穩(wěn)定均一的組織裂解液，利用固相萃取法和磁珠篩選法配合使用獲得高質(zhì)量高濃度的動物樣品DNA。

In preclinical research of stem cell drugs, it was often necessary to track the distribution process of stem cell specific gene fragments in vivo, and extract genomic DNA of animal samples, involving heart, liver, lung, spleen, kidney, brain, bone marrow, blood, fat, muscle, gonad, stomach and intestine. For such a special purpose of DNA extraction, there was no systematic report. In this paper, the methods of extracting genomic DNA from animal tissues were summarized, and how to extract genomic DNA from samples with high efficiency and high quality was discussed. A feasible scheme for sample treatment in preclinical research of mesenchymal stem cells was proposed:stable and uniform tissue lysate was obtained by biological sample preparation apparatus, and high quality and high concentration animal sample DNA was obtained by solid phase extraction and magnetic bead screening.

天津市科技計劃項目（17ZXXYSY00020）