目的 觀察人源臍帶間充質(zhì)干細(xì)胞（hUC-MSCs）對異體總淋巴細(xì)胞和不同淋巴細(xì)胞亞群增殖能力的影響，以及對細(xì)胞因子分泌的影響。方法 分離并培養(yǎng)hUC-MSCs及健康成人外周血淋巴細(xì)胞，以淋巴細(xì)胞為陰性對照，MTT法檢測共培養(yǎng)（淋巴細(xì)胞：hUC-MSCs為1：0.5、1：1、1：5、1：10）3 d后淋巴細(xì)胞的增殖情況；流式細(xì)胞儀檢測共培養(yǎng)體系中CD4⁺CD25⁺FOXP3⁺、CD3^-CD16⁺CD56⁺、CD4⁺CD45RA⁺CCR7⁺的細(xì)胞比例；ELISA法檢測白細(xì)胞介素（IL）-2、IL-4、IL-10、γ-干擾素（INF-γ）細(xì)胞因子分泌情況。結(jié)果 與陰性對照組比較，hUC-MSCs對淋巴細(xì)胞的增殖具有顯著抑制作用（P<0.05），抑制效果隨數(shù)量增加而增強(qiáng)；hUC-MSCs能夠顯著增加共培養(yǎng)體系中CD4⁺CD25⁺FOXP3⁺細(xì)胞比例（P<0.05），顯著降低CD4⁺CD45RA⁺CCR7⁺細(xì)胞比例（P<0.05），對CD3^-CD16⁺CD56⁺細(xì)胞比例無顯著影響；hUC-MSCs能夠顯著降低IL-2、IL-4、INF-γ水平，顯著增加IL-10的分泌水平（P<0.05）。結(jié)論 hUC-MSCs具有免疫抑制功能。

Objective To observe the effects of human umbilical cord mesenchymal stem cells (hUC-MSCs) on the proliferation of allogeneic total lymphocytes and different lymphocyte subsets, as well as the secretion of cytokines. Methods hUC-MSCs and peripheral blood lymphocytes of healthy adults were isolated and cultured; MTT method was used to detect the proliferation of lymphocytes by after co-culture for 3 days; the proportion of CD4⁺CD25⁺FOXP3⁺, CD3^-CD16⁺CD56⁺ and CD4⁺CD45RA⁺CCR7⁺ in the co-culture system was detected by flow cytometry; ELISA was used to detect the secretion of IL-2, IL-4, IL-10 and INF-γ. Results hUC-MSCs could significant inhibit the proliferation of T lymphocytes, and the anti-proliferative effect was enhanced with the increase of the number of lymphocytes; the proportion of CD4⁺CD25⁺FOXP3⁺ cells in co-culture system was increased by hUCMSCs (P<0.05); the proportion of CD4⁺CD45RA⁺CCR7⁺ cells showed a downward tendency, and the difference was statistically significant (P<0.05); however, there was no significant difference in CD3^-CD16⁺CD56⁺ cells. hUC-MSCs could reduce the levels of IL-2, IL-4 and INF-γ, but increase the secretion level of IL-10, and the difference was statistically significant (P<0.05). Conclusion HUC-MSCs had immunosuppressive function.

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國家重點(diǎn)研發(fā)計(jì)劃“干細(xì)胞及轉(zhuǎn)化研究”重點(diǎn)專項(xiàng)（2019YFA0112100）；天津市科技計(jì)劃項(xiàng)目（17ZXXYSY00020）